US 12,421,615 B2
Engineered electrode for electrobiocatalysis and process to construct the same
Manoj Kumar, Haryana (IN); Prakash Chandra Sahoo, Haryana (IN); Srikanth Sandipam, Haryana (IN); Suresh Kumar Puri, Haryana (IN); and Sankara Sri Venkata Ramakumar, Haryana (IN)
Assigned to Indian Oil Corporation Limited, Mumbai (IN)
Filed by Indian Oil Corporation Limited, Mumbai (IN)
Filed on Mar. 13, 2024, as Appl. No. 18/603,491.
Application 18/603,491 is a division of application No. 16/668,832, filed on Oct. 30, 2019, abandoned.
Claims priority of application No. 201821040950 (IN), filed on Oct. 30, 2018.
Prior Publication US 2024/0218537 A1, Jul. 4, 2024
Int. Cl. C25B 11/073 (2021.01); B82Y 5/00 (2011.01); B82Y 15/00 (2011.01); B82Y 30/00 (2011.01); C12N 1/20 (2006.01); C12N 11/02 (2006.01); C25B 3/00 (2021.01); C25B 11/043 (2021.01); H01M 8/16 (2006.01)
CPC C25B 11/073 (2021.01) [C12N 1/20 (2013.01); C12N 11/02 (2013.01); C25B 3/00 (2013.01); C25B 11/043 (2021.01); H01M 8/16 (2013.01); B82Y 5/00 (2013.01); B82Y 15/00 (2013.01); B82Y 30/00 (2013.01)] 9 Claims
 
1. A process for constructing a bio-electrode, the process consisting of:
selecting an electro-active bacteria from the group consisting of Enterobacter aerogenes MTCC 25016, Serratia sp. MTCC 25017, Citrobacter intermedius MTCC 25018, Pseudomonas aeruginosa MTCC 25019, Shewanella sp. MTCC 25020, Pseudomonas stutzeri MTCC 25027, Clostridium ljungdahlii DSM 13528, Sporomusa silvacetica DSM 10669, Cupriavidus nectar DSM 529, Sporomosa ovate DSM 2662, and combinations thereof;
enriching the electro-active bacteria in a nutrient rich medium to obtain electro-active bacterial cells;
separating the electro-active bacterial cells obtained from the nutrient rich medium;
providing an electrode material;
surface modifying the electrode material by treating it with an acid and rinsing with a bicarbonate buffer;
layering the surface modified electrode material with a conductive material and drying for a period of 1-4 hours at room temperature;
further layering the dried surface modified electrode material with the electro-active bacterial cells, adding a biofilm inducing agent, drying at room temperature for a period of 45 minutes, and applying a stabilizing agent;
conditioning the electro-active bacterial cells layered surface modified electrode material; and
incubating the conditioned electro-active bacterial cells layered surface modified electrode material with an immobilizing agent and the conductive material for a period of 2-3 hours at 30° C., and conditioning with micronutrients to obtain the bio-electrode,
the constructing of the bio-electrode takes a time period of 8 to 24 hours to impart a shelf-life of at least 2-3 years.