US 12,421,539 B2
Methods and compositions for nucleic acid analysis
Xinying Zheng, San Jose, CA (US); Serge Saxonov, Oakland, CA (US); Michael Schnall-Levin, San Francisco, CA (US); Kevin Ness, Pleasanton, CA (US); and Rajiv Bharadwaj, Pleasanton, CA (US)
Assigned to 10x Genomics, Inc., Pleasanton, CA (US)
Filed by 10X GENOMICS, INC., Pleasanton, CA (US)
Filed on Nov. 27, 2023, as Appl. No. 18/520,514.
Application 18/520,514 is a continuation of application No. 17/943,064, filed on Sep. 12, 2022, granted, now 11,873,528.
Application 17/943,064 is a continuation of application No. 16/191,343, filed on Nov. 14, 2018, granted, now 11,473,125.
Application 16/191,343 is a continuation of application No. 15/367,660, filed on Dec. 2, 2016, granted, now 10,774,370.
Claims priority of provisional application 62/263,532, filed on Dec. 4, 2015.
Prior Publication US 2024/0167079 A1, May 23, 2024
This patent is subject to a terminal disclaimer.
Int. Cl. C12Q 1/6809 (2018.01); C12Q 1/6806 (2018.01); C12Q 1/6834 (2018.01); C12Q 1/6869 (2018.01)
CPC C12Q 1/6809 (2013.01) [C12Q 1/6806 (2013.01); C12Q 1/6834 (2013.01); C12Q 1/6869 (2013.01)] 15 Claims
 
1. A method of analyzing nucleic acids in a formalin fixed paraffin embedded (FFPE) tissue sample while maintaining spatial context, the method comprising:
(a) providing a FFPE tissue sample comprising nucleic acids;
(b) applying a library of tags to the tissue sample such that different geographical regions of the tissue sample receive different tags or different concentrations of tags;
(c) following (b), separating portions of the tissue sample into discrete partitions such that portions of the library of tags and portions of the nucleic acids are also separated into the discrete partitions;
(d) obtaining sequence information from the nucleic acids, and
(e) identifying tags or concentrations of tags in the discrete partitions, thereby analyzing said nucleic acids while maintaining spatial context.