US 12,421,520 B2
Modified bidirectional catalase promoter from bacillus
Max Fabian Felle, Ludwigshafen (DE); Stefan Jenewein, Ludwigshafen (DE); Kerstin Hage, Nienburg (DE); and Holger Hartmann, Ludwigshafen (DE)
Assigned to BASF SE, Ludwigshafen am Rhein (DE)
Filed by BASF SE, Ludwigshafen (DE)
Filed on Sep. 8, 2023, as Appl. No. 18/463,923.
Application 18/463,923 is a division of application No. 16/062,291, filed on Jun. 14, 2018, granted, now 11,788,094.
Prior Publication US 2024/0093211 A1, Mar. 21, 2024
Int. Cl. C12N 15/75 (2006.01); C12N 9/08 (2006.01); C12N 15/63 (2006.01); C12P 1/04 (2006.01)
CPC C12N 15/75 (2013.01) [C12N 9/0065 (2013.01); C12N 15/63 (2013.01); C12P 1/04 (2013.01); C12Y 111/01006 (2013.01)] 11 Claims
 
1. A promoter comprising a −10 type box and a −35 type box separated from one another by a linker section,
wherein each box consists of a respective nucleotide sequence with a respective score obtainable by assigning a value to each nucleotide at each position according to table 1 for the −10 type box:
TABLE 1
 
position (5′->3′
 
 
 
 
direction A C G T
 
 
1 0 0 0 100
2 0 0 100 0
3 28 6 30 36
4 8 5 0 88
5 94 0 2 5
6 23 11 6 59
7 73 5 6 16
8 63 20 3 14
9 2 2 2 95
 
and according to table 2 for the −35 type box:
TABLE 2
 
position (5′->3′
 
 
 
 
direction A C G T
 
 
1 2 3 0 95
2 6 3 2 89
3 2 8 81 9
4 63 16 3 19
5 20 58 5 17
6 53 8 11 28
 
wherein the score for the −10 type box is at least 497 and for the −35 type box is at least 179, and wherein the linker section has a length of at least 14 nucleotides and an A/T content of at least 57%, and
wherein the nucleotide sequence of the linker section differs from any of SEQ ID NOS: 3 to 18 by insertion of a thymidine before any of nucleotide positions 1 to 5 of these sequences.