US 12,421,509 B2
Engineered phosphopentomutase variant enzymes
Scott J. Novick, Palo Alto, CA (US); Xiang Yi, San Carlos, CA (US); Nikki Dellas, San Carlos, CA (US); Oscar Alvizo, Fremont, CA (US); Jovana Nazor, Milpitas, CA (US); Da Duan, Foster City, CA (US); Vesna Mitchell, Santa Clara, CA (US); Jonathan Vroom, South San Francisco, CA (US); Santhosh Sivaramakrishnan, Redwood City, CA (US); Nandhitha Subramanian, Milton Keynes (GB); Jeffrey C. Moore, Westfield, NJ (US); Mark Huffman, Rahway, NJ (US); Agustina Rodriguez-Granillo, Rahway, NJ (US); Deeptak Verma, Rahway, NJ (US); Grant S. Murphy, Rahway, NJ (US); Nicholas Marshall, Rahway, NJ (US); Jay Russell, Rahway, NJ (US); and Keith A. Canada, Freehold, NJ (US)
Assigned to Codexis, Inc., Redwood City, CA (US)
Filed by Codexis, Inc., Redwood City, CA (US)
Filed on Sep. 14, 2023, as Appl. No. 18/467,259.
Application 17/316,165 is a division of application No. 16/460,062, filed on Jul. 2, 2019, granted, now 11,034,948, issued on Jun. 15, 2021.
Application 18/467,259 is a continuation of application No. 17/316,165, filed on May 10, 2021, granted, now 11,795,445.
Claims priority of provisional application 62/695,491, filed on Jul. 9, 2018.
Prior Publication US 2024/0060064 A1, Feb. 22, 2024
Int. Cl. C12N 9/90 (2006.01); C12N 15/52 (2006.01); C12N 15/74 (2006.01); C12N 15/81 (2006.01)
CPC C12N 9/90 (2013.01) [C12N 15/52 (2013.01); C12N 15/74 (2013.01); C12N 15/815 (2013.01); C12Y 504/02007 (2013.01)] 18 Claims
 
1. An engineered phosphopentomutase comprising a polypeptide sequence having at least 85% sequence identity to SEQ ID NO: 2, wherein the polypeptide sequence of said engineered phosphopentomutase comprises at least one substitution or substitution set relative to SEQ ID NO: 2 at one or more positions in said polypeptide sequence selected from 98, 64/98/99/146/179/231/261/357, 98/99/146/231/357, 98/99/146/179/261/357/397, 98/99/146/153/231/261/357, 98/99/146/231, 98/99/146/179/231/261/357, 98/99/231/261/357/397, 98/99/146/261/357/397, 98/99/146/153/231/357, 98/99/146/153/231/357, 98/99/146/179/231/261/357/397, 98/99/146/231/261/357/397, 98/99/146/231/261/357, 98/99/146/179/231/261/357/397, 98/99/146/261/357/397, 98/99/146/179/231/357, 98/99/146/231/357, 98/99/146/153/179/231/357, and 98/99/146/153/231/261/357/397.