US 12,421,503 B2
Ketoreductase polypeptides for the production of azetidinone
Onorato Campopiano, Hayward, CA (US); Emily Mundorff, Garden City, NY (US); Birthe Borup, Wesel (DE); and Rama Voladri, Pleasanton, CA (US)
Assigned to Codexis, Inc., Redwood City, CA (US)
Filed by Codexis, Inc., Redwood City, CA (US)
Filed on Sep. 22, 2023, as Appl. No. 18/472,540.
Application 16/790,474 is a division of application No. 16/404,988, filed on May 7, 2019, granted, now 10,597,641, issued on Mar. 24, 2020.
Application 16/404,988 is a division of application No. 15/631,359, filed on Jun. 23, 2017, granted, now 10,329,540, issued on Jun. 25, 2019.
Application 15/272,664 is a division of application No. 15/175,317, filed on Jun. 7, 2016, granted, now 9,476,034, issued on Oct. 25, 2016.
Application 13/569,900 is a division of application No. 12/977,825, filed on Dec. 23, 2010, granted, now 8,257,952, issued on Sep. 4, 2012.
Application 18/472,540 is a continuation of application No. 17/357,673, filed on Jun. 24, 2021, granted, now 12,049,649.
Application 17/357,673 is a continuation of application No. 16/790,474, filed on Feb. 13, 2020, granted, now 11,078,466, issued on Aug. 3, 2021.
Application 15/631,359 is a continuation of application No. 15/408,673, filed on Jan. 18, 2017, granted, now 9,719,071, issued on Mar. 1, 2017.
Application 15/408,673 is a continuation of application No. 15/272,664, filed on Sep. 22, 2016, granted, now 9,580,694, issued on Feb. 28, 2017.
Application 15/175,317 is a continuation of application No. 14/822,624, filed on Aug. 10, 2015, granted, now 9,382,519, issued on Jul. 5, 2016.
Application 14/822,624 is a continuation of application No. 14/658,407, filed on Mar. 16, 2015, granted, now 9,133,442, issued on Sep. 15, 2015.
Application 14/658,407 is a continuation of application No. 13/925,096, filed on Jun. 24, 2013, granted, now 8,980,606, issued on Mar. 17, 2015.
Application 13/925,096 is a continuation of application No. 13/569,900, filed on Aug. 8, 2012, granted, now 8,470,572, issued on Jun. 25, 2013.
Application 12/977,825 is a continuation of application No. 12/243,968, filed on Oct. 1, 2008, granted, now 7,883,879, issued on Feb. 8, 2011.
Claims priority of provisional application 60/976,555, filed on Oct. 1, 2007.
Prior Publication US 2024/0141304 A1, May 2, 2024
This patent is subject to a terminal disclaimer.
Int. Cl. C12N 9/04 (2006.01); C12P 17/10 (2006.01)
CPC C12N 9/0006 (2013.01) [C12P 17/10 (2013.01); C12Y 101/01184 (2013.01); Y02P 20/52 (2015.11)] 14 Claims
 
1. An engineered ketoreductase polypeptide having ketoreductase activity,
wherein said ketoreductase polypeptide is at least 85% identical to SEQ ID NO:4, and
wherein the ketoreductase polypeptide comprises the following fetures:
the resiude at the position corresponding to position 153 is alanine or serine,
the residue at position corresponding to position 202 is valine or leucine, and
wherein the residue at the position corresponding to position 94 is threonine.