US 12,421,486 B2
Method to increase the permeability of the plasma membrane of cells and a structure suitable for use in such method
Kevin Braeckmans, Lokeren (BE); Ranhua Xiong, Ghent (BE); and Stefaan De Smedt, Mariakerke (BE)
Assigned to Trince BV, Ghent (BE)
Appl. No. 17/753,868
Filed by Trince BV, Ghent (BE)
PCT Filed Sep. 21, 2020, PCT No. PCT/EP2020/076287
§ 371(c)(1), (2) Date Mar. 16, 2022,
PCT Pub. No. WO2021/058430, PCT Pub. Date Apr. 1, 2021.
Claims priority of application No. 19198937 (EP), filed on Sep. 23, 2019.
Prior Publication US 2022/0403317 A1, Dec. 22, 2022
Int. Cl. C12M 1/42 (2006.01); C12N 13/00 (2006.01)
CPC C12M 35/02 (2013.01) [C12N 13/00 (2013.01)] 11 Claims
OG exemplary drawing
 
1. A method to increase the permeability of the plasma membrane of cells, said method comprising the steps of
providing a structure comprising a material and comprising particles able to absorb electromagnetic radiation embedded in said material, said particles having an average equivalent spherical diameter d, said structure defining a volume V and a free area surface S, said particles being present in said structure in a concentration ranging between 0.001 vol % and 20 vol % (volume particles/volume structure), at least P percent of said particles present in said structure being embedded in said material in such a way that the shortest distance L between said P percent of said particles and said free area surface S of said structure ranges between 1 nm and 500 nm; P being at least 60%,
introducing at least one cell on or at a distance of less than 100 μm from said structure; irradiating said structure with electromagnetic radiation.