US 12,421,483 B2
Biofabrication system
Lester J. Smith, Maud, TX (US)
Assigned to THE TRUSTEES OF INDIANA UNIVERSITY, Bloomington, IN (US)
Appl. No. 17/599,838
Filed by THE TRUSTEES OF INDIANA UNIVERSITY, Indianapolis, IN (US)
PCT Filed Apr. 3, 2020, PCT No. PCT/US2020/026632
§ 371(c)(1), (2) Date Sep. 29, 2021,
PCT Pub. No. WO2020/210126, PCT Pub. Date Oct. 15, 2020.
Claims priority of provisional application 62/831,366, filed on Apr. 9, 2019.
Prior Publication US 2022/0135922 A1, May 5, 2022
Int. Cl. C12M 3/00 (2006.01); C12M 1/00 (2006.01); C12M 1/12 (2006.01); C12M 1/32 (2006.01); C12N 5/00 (2006.01)
CPC C12M 21/08 (2013.01) [C12M 23/12 (2013.01); C12M 23/34 (2013.01); C12M 23/38 (2013.01); C12M 23/48 (2013.01); C12M 25/02 (2013.01); C12M 29/10 (2013.01); C12M 29/20 (2013.01); C12M 47/02 (2013.01); C12N 5/0068 (2013.01)] 15 Claims
OG exemplary drawing
 
1. A biofabrication system comprising
a production container including
a production floor having a top surface and a bottom surface,
a production wall extending from the top surface of the production floor, wherein the production floor and the production wall define a production reservoir,
a formation section defined in the top surface of the production floor, wherein the formation section comprises a formation well, wherein said formation well comprises a formation wall and a formation floor, optionally wherein the formation floor is concave, and
a grip extending outwardly from the formation floor through said formation section and into the production reservoir;
a capture container including
a capture foundation having a top surface and a bottom surface, wherein the top surface is configured to define a conically shaped capture reservoir having a vertex, and the bottom surface defines a capture channel;
wherein said capture foundation is configured to be positioned to contact the production container for transfer of material formed in the production container to the capture container;
a platen and an undercast, the platen and the undercast configured for receiving the material transferred to the capture container to form a tissue construct therein,
the platen including a platen top surface and a platen bottom surface, wherein
(i) said platen top surface is configured to define a conically shaped platen reservoir having a vertex with a flat surface at a bottom of said platen reservoir, wherein said flat surface comprises at least one platen opening allowing fluid communication from the platen top surface and the platen bottom surface, and
(ii) said platen bottom surface is configured to define a platen channel;
the undercast configured to be coupled with the platen and including
an undercast floor comprising a relief port;
an undercast wall extending outwardly from a perimeter of said undercast floor;
an undercast grip extending from the undercast floor and concentric to said undercast wall to define a perfusible-formation section and an undercast member that extends outwardly from the perfusible-formation section and aligns with said at least one platen opening when said platen is coupled to said undercast; and
a bioreactor configured to receive the platen and the tissue construct therein, the bioreactor including:
(i) a bioreactor base having a bioreactor well defined therein; wherein the bioreactor well has a well floor and a well wall,
(ii) an inlet port in fluid communication with said bioreactor well, and
(iii) an outlet port in fluid communication with said bioreactor well,
wherein said production container, said capture container, said platen, said undercast, and said bioreactor are each made of a biocompatible material that remains stable when autoclaved.