US 12,421,472 B2
Process for producing extracted lipid comprising docosahexaenoic acid
James Robertson Petrie, Goulburn (AU); Surinder Pal Singh, Downer (AU); and Robert Charles de Feyter, Monash (AU)
Assigned to COMMONWEALTH SCIENTIFIC INDUSTRIAL RESEARCH ORGANISATION -, Acton (AU); and GRAINS RESEARCH AND DEVELOPMENT CORPORATION - Barton, Australia, Laverton (AU)
Filed by Commonwealth Scientific and Industrial Research Organisation, Acton (AU); Grains Research and Development Corporation, Barton (AU); and Nuseed Nutritional Australia Pty Ltd, Laverton North (AU)
Filed on Mar. 30, 2022, as Appl. No. 17/709,128.
Application 17/709,128 is a continuation of application No. 16/868,324, filed on May 6, 2020, granted, now 11,306,271.
Application 16/868,324 is a continuation of application No. 15/961,200, filed on Apr. 24, 2018, granted, now 10,655,082, issued on May 19, 2020.
Application 15/961,200 is a continuation of application No. 15/678,008, filed on Aug. 15, 2017, granted, now 9,969,954, issued on May 15, 2018.
Application 15/678,008 is a continuation of application No. 15/400,532, filed on Jan. 6, 2017, granted, now 9,932,289, issued on Apr. 3, 2018.
Application 15/400,532 is a continuation of application No. 14/600,653, filed on Jan. 20, 2015, granted, now 9,556,102, issued on Jan. 31, 2017.
Application 14/600,653 is a continuation of application No. 13/918,392, filed on Jun. 14, 2013, granted, now 8,946,460, issued on Feb. 3, 2015.
Claims priority of provisional application 61/782,680, filed on Mar. 14, 2013.
Claims priority of provisional application 61/697,676, filed on Sep. 6, 2012.
Claims priority of provisional application 61/663,344, filed on Jun. 22, 2012.
Claims priority of provisional application 61/660,392, filed on Jun. 15, 2012.
Prior Publication US 2022/0356414 A1, Nov. 10, 2022
This patent is subject to a terminal disclaimer.
Int. Cl. C11B 1/10 (2006.01); A01H 1/00 (2006.01); A01H 5/10 (2018.01); A01H 6/20 (2018.01); A23K 20/158 (2016.01); A23L 33/12 (2016.01); A61K 31/231 (2006.01); A61K 31/232 (2006.01); A61K 36/31 (2006.01); C07C 57/02 (2006.01); C07C 57/03 (2006.01); C07C 67/02 (2006.01); C07K 14/415 (2006.01); C11B 1/00 (2006.01); C11C 1/00 (2006.01); C11C 3/00 (2006.01); C11C 3/06 (2006.01); C12N 9/10 (2006.01); C12N 15/82 (2006.01)
CPC C11B 1/10 (2013.01) [A01H 1/104 (2021.01); A01H 5/10 (2013.01); A01H 6/20 (2018.05); A61K 36/31 (2013.01); C07C 57/02 (2013.01); C07C 57/03 (2013.01); C07C 67/02 (2013.01); C11B 1/00 (2013.01); C11C 1/002 (2013.01); C11C 3/00 (2013.01); C11C 3/003 (2013.01); C11C 3/06 (2013.01); C12N 9/1025 (2013.01); C12N 15/8247 (2013.01); A23K 20/158 (2016.05); A23L 33/12 (2016.08); A23V 2002/00 (2013.01); A61K 31/231 (2013.01); A61K 31/232 (2013.01); A61K 2236/35 (2013.01); C07K 14/415 (2013.01)] 13 Claims
 
1. A process for producing extracted plant lipid comprising the steps of:
i) obtaining Brassica napus seed or Camelina sativa seed from Brassica napus plants or Camelina sativa plants, respectively, wherein the plants are grown in a field as a population of at least 1,000 such plants, which seed comprise extractable lipid having a total fatty acid content comprising fatty acids in an esterified form, the fatty acids comprising oleic acid, palmitic acid, a total ω6 fatty acid content which comprises linoleic acid (LA) and γ-linolenic acid (GLA), and a total ω3 fatty acid content which comprises α-linolenic acid (ALA), stearidonic acid (SDA), docosapentaenoic acid (DPA), eicosapentaenoic acid (EPA) and eicosatetraenoic acid (ETA), wherein
a) palmitic acid is present in the extractable lipid at a level of between 2% and 16% of the total fatty acid content,
b) myristic acid is either absent or is present in the extractable lipid at a level of less than 1% of the total fatty acid content,
c) oleic acid is present in the extractable lipid at a level of between 1% and 30% of the total fatty acid content,
d) LA is present in the extractable lipid at a level of between 4% and 35% of the total fatty acid content,
e) GLA is present in the extractable lipid at a level of less than 4% of the total fatty acid content,
f) ALA is present in the extractable lipid at a level of between 4% and 40% of the total fatty acid content,
g) the total saturated fatty acid content of the extractable lipid is between 4% and 25% of the total fatty acid content,
h) the ratio of the total ω6 fatty acid content to the total ω3 fatty acid content in the total fatty acid content is between 0.1 and 1, and
i) SDA, ETA, EPA and DPA are each present at a level in the total fatty acid content, each level being expressed as a percentage of the total fatty acid content, whereby the sum of the percentages for ETA, EPA and DPA divided by the sum of the percentages for SDA, ETA, EPA and DPA, expressed as a percentage, is at least 75%,
wherein each percentage is a percentage by weight,
and
ii) extracting lipid from the seed to thereby produce the extracted plant lipid.