	US 12,421,299 B2
	Expression of FC-containing proteins
Benjamin Haley, San Francisco, CA (US); Zhilan Hu, Foster City, CA (US); John C. Joly, San Mateo, CA (US); Amy Y. Shen, San Mateo, CA (US); and Bradley Richard Snedecor, Portola Valley, CA (US)
Assigned to GENENTECH, INC., South San Francisco, CA (US)
Filed by Genentech, Inc., South San Francisco, CA (US)
Filed on Jan. 18, 2023, as Appl. No. 18/156,215.
Application 18/156,215 is a division of application No. 15/761,887, granted, now 11,591,382, previously published as PCT/US2016/052962, filed on Sep. 21, 2016.
Claims priority of provisional application 62/222,187, filed on Sep. 22, 2015.
Prior Publication US 2023/0159625 A1, May 25, 2023
This patent is subject to a terminal disclaimer.
Int. Cl. C07K 16/00 (2006.01); C12N 5/00 (2006.01); C12N 15/113 (2010.01); C12N 15/85 (2006.01); C12P 21/02 (2006.01)

CPC C07K 16/00 (2013.01) [C12N 15/1137 (2013.01); C12N 15/85 (2013.01); C12P 21/02 (2013.01); C07K 2317/14 (2013.01); C07K 2317/52 (2013.01); C07K 2319/30 (2013.01); C12N 2310/14 (2013.01); C12N 2310/20 (2017.05); C12N 2510/00 (2013.01); C12N 2510/02 (2013.01); C12N 2511/00 (2013.01)]

13 Claims

1. A cell culture comprising:

a plurality of Fc-containing proteins,

wherein substantially all Fc-containing proteins in the cell culture have a C-terminal lysine on each Fc domain; and

a mammalian host cell engineered to express the plurality of Fc-containing proteins,

wherein the mammalian host cell has carboxypeptidase D (CpD) gene knocked out and CpD is the only carboxypeptidase gene knocked out, and

wherein the mammalian host cell comprises an expression vector comprising a nucleic acid encoding the Fc-containing protein comprising the C-terminal lysine.