US 12,421,277 B2
Anti-aging salmon roe tripeptide and preparation method and application thereof
Tiantian Zhao, Shenzhen (CN)
Assigned to Shenzhen Porshealth Bioengineering Co., Ltd., Shenzhen (CN)
Filed by Shenzhen Porshealth Bioengineering Co., Ltd., Shenzhen (CN)
Filed on May 1, 2024, as Appl. No. 18/651,979.
Claims priority of application No. 202311694473.1 (CN), filed on Dec. 12, 2023.
Prior Publication US 2024/0279276 A1, Aug. 22, 2024
Int. Cl. C12P 21/06 (2006.01); A61K 38/00 (2006.01); C07K 5/087 (2006.01)
CPC C07K 5/0812 (2013.01) [C12P 21/06 (2013.01); A61K 38/00 (2013.01)] 3 Claims
 
1. A preparation method of a salmon roe tripeptide that increases the content of the collagen and hyaluronic acid, wherein a peptide sequence is YLP and the method specifically comprises the following steps:
(1) adding deionized water to salmon roe for homogenization to obtain a salmon roe homogenate;
wherein a mass ratio of the salmon roe to the deionized water is 1:6; for homogenization, a rotating speed is 8000 rpm, and a time is 1 min;
(2) regulating a pH value of the salmon roe homogenate, and adding pancreatin for enzymolysis, enzyme inactivation and cooling to obtain a salmon roe enzymatic hydrolysate;
wherein an addition amount of the pancreatin is 1%;
for enzymolysis, a temperature is 55° C., and a time is 4 h;
(3) centrifuging the salmon roe enzymatic hydrolysate, taking a supernatant which is the anti-aging salmon roe tripeptide, and storing the anti-aging salmon roe tripeptide for later use;
wherein for centrifugation treatment, a temperature is 4° C.; a rotating speed is 8000 rpm; and
a time is 15 min.