US 12,420,262 B2
Chromatographic column having stationary phase thickness gradient
Xudong Fan, Saline, MI (US); Hongbo Zhu, Johnson City, TN (US); Jinyan She, Ann Arbor, MI (US); Maxwell Wei-Hao Li, Troy, MI (US); and Katsuo Kurabayashi, Ann Arbor, MI (US)
Assigned to The Regents of The University of Michigan, Ann Arbor, MI (US)
Appl. No. 17/779,247
Filed by THE REGENTS OF THE UNIVERSITY OF MICHIGAN, Ann Arbor, MI (US)
PCT Filed Nov. 25, 2020, PCT No. PCT/US2020/062266
§ 371(c)(1), (2) Date May 24, 2022,
PCT Pub. No. WO2021/137979, PCT Pub. Date Jul. 8, 2021.
Claims priority of provisional application 62/940,038, filed on Nov. 25, 2019.
Prior Publication US 2022/0410122 A1, Dec. 29, 2022
Int. Cl. B01L 3/00 (2006.01); B01D 15/08 (2006.01); B01F 33/302 (2022.01); B01F 33/3033 (2022.01); B01J 20/285 (2006.01); B01J 20/287 (2006.01); B01J 20/32 (2006.01); B01L 7/00 (2006.01); B01L 9/00 (2006.01); B65G 47/80 (2006.01); B82Y 20/00 (2011.01); B82Y 30/00 (2011.01); B82Y 40/00 (2011.01); C12M 1/34 (2006.01); C12M 3/06 (2006.01); C12N 1/14 (2006.01); C12N 1/20 (2006.01); C12Q 1/02 (2006.01); C12Q 1/6806 (2018.01); C12Q 1/6844 (2018.01); C12Q 1/6848 (2018.01); C12Q 1/686 (2018.01); G01N 15/10 (2024.01); G01N 15/14 (2024.01); G01N 15/1433 (2024.01); G01N 21/29 (2006.01); G01N 21/65 (2006.01); G01N 30/02 (2006.01); G01N 30/60 (2006.01); G01N 30/72 (2006.01); G01N 33/543 (2006.01); G01N 33/557 (2006.01); G01N 33/574 (2006.01); G01N 33/58 (2006.01)
CPC B01J 20/287 (2013.01) [B01J 20/285 (2013.01); B01J 20/3282 (2013.01); G01N 30/6065 (2013.01); G01N 30/6078 (2013.01); G01N 2030/025 (2013.01)] 18 Claims
OG exemplary drawing
 
9. A method of peak focusing in a gas chromatography device, comprising:
introducing two or more target analytes into an inlet of a chromatographic column comprising a stationary phase deposited inside the chromatographic column and having a positive thickness gradient, wherein the stationary phase extends from the inlet to an outlet and has a first thickness at the inlet of the chromatographic column and a second thickness at the outlet of the chromatographic column, wherein the second thickness is at least about 10% greater than the first thickness;
separating two or more target analytes in the chromatographic column; and
eluting the two or more target analytes from the outlet of the chromatographic column.