US 12,419,993 B2
Cardiac fibroblast derived extracellular matrix
Eric Schmuck, Madison, WI (US); Amish Raval, Madison, WI (US); Sushmita Roy, Madison, WI (US); and Tianhua Zhou, Madison, WI (US)
Assigned to Wisconsin Alumni Research Foundation
Filed by WISCONSIN ALUMNI RESEARCH FOUNDATION, Madison, WI (US)
Filed on Sep. 6, 2022, as Appl. No. 17/929,890.
Claims priority of provisional application 63/241,240, filed on Sep. 7, 2021.
Prior Publication US 2023/0072362 A1, Mar. 9, 2023
Int. Cl. A61L 27/36 (2006.01); A61K 35/12 (2015.01); A61L 27/38 (2006.01); C12N 5/077 (2010.01)
CPC A61L 27/3633 (2013.01) [A61K 35/12 (2013.01); C12N 5/0657 (2013.01); A61L 27/3834 (2013.01); C12N 2533/90 (2013.01)] 22 Claims
OG exemplary drawing
 
1. A method for preparing a cardiac extracellular matrix, comprising:
(a) selecting SUSD2 High fibroblasts from a population of cardiac fibroblasts; and
(b) plating the SUSD2 High fibroblasts into a culture having a cell density of 100,000 to 500,000 cells per cm2, wherein the SUSD2 High fibroblasts secrete a cardiac extracellular matrix that is attached to the surface on which the SUSD2 High fibroblasts are plated.