US 12,419,992 B2
Cartilage matrix
Alexander Huber, Chesapeake, VA (US); and Paresa Taghavie-Moghadam, Virginia Beach, VA (US)
Assigned to Matney Legal Group PLLC, Virginia Beach, VA (US)
Appl. No. 16/631,942
Filed by LifeNet Health, Virginia Beach, VA (US)
PCT Filed Jul. 20, 2018, PCT No. PCT/US2018/043144
§ 371(c)(1), (2) Date Jan. 17, 2020,
PCT Pub. No. WO2019/018800, PCT Pub. Date Jan. 24, 2019.
Claims priority of provisional application 62/535,151, filed on Jul. 20, 2017.
Prior Publication US 2020/0222588 A1, Jul. 16, 2020
Int. Cl. A61L 27/36 (2006.01); A61L 27/38 (2006.01)
CPC A61L 27/3612 (2013.01) [A61L 27/3687 (2013.01); A61L 27/3817 (2013.01); A61L 27/3852 (2013.01); A61L 27/3895 (2013.01); A61L 2300/406 (2013.01); A61L 2300/414 (2013.01)] 16 Claims
OG exemplary drawing
 
1. An implant comprising a composition, wherein the composition comprises a cartilage matrix putty comprising dehydrated, deglycosylated, decellularized cartilage matrix particles and water, wherein the cartilage matrix putty is prepared according to a method comprising:
(a) incubating a cartilage in a decellularization solution comprising a detergent, whereby a decellularized cartilage matrix is generated in the form of particles;
(b) deglycosylating the decellularized cartilage matrix particles, comprising treating the particles with a deglycosylation solution comprising a glycolytic enzyme, a proteolytic enzyme, an acid, hydrazine, or a combination thereof, and dehydrating the deglycosylated, decellularized cartilage matrix particles; and
(c) rehydrating the dehydrated, deglycosylated, decellularized cartilage matrix particles with a solution comprising water to yield a homogenous cartilage matrix putty;
wherein the cartilage matrix putty is cohesive, and wherein cohesive is defined as:
when a sample of the cartilage matrix putty consisting of the dehydrated, deglycosylated, decellularized cartilage matrix particles and 1 mL water per 2 grams of the dehydrated, deglycosylated, decellularized cartilage matrix particles is placed in an aqueous environment the sample of the cartilage matrix putty retains at least 95% of its weight after 180 minutes in the aqueous environment.