US 12,419,948 B2
Immunogenic compositions and use thereof
Pardis Sabeti, Cambridge, MA (US); Shira Weingarten-Gabbay, Cambridge, MA (US); Susan Klaeger, Cambridge, MA (US); Jenn Abelin, Cambridge, MA (US); Mohsan Saeed, Boston, MA (US); Nir Hacohen, Boston, MA (US); Siranush Sarkizova, Cambridge, MA (US); Steven Carr, Cambridge, MA (US); and Karl Clauser, Cambridge, MA (US)
Assigned to The Broad Institute, Inc., Cambridge, MA (US); President and Fellows of Harvard College, Cambridge, MA (US); The General Hospital Corporation, Boston, MA (US); and Trustees of Boston University, Boston, MA (US)
Filed by THE BROAD INSTITUTE, INC., Cambridge, MA (US); PRESIDENT AND FELLOWS OF HARVARD COLLEGE, Cambridge, MA (US); THE GENERAL HOSPITAL CORPORATION, Boston, MA (US); and TRUSTEES OF BOSTON UNIVERSITY, Boston, MA (US)
Filed on Aug. 31, 2021, as Appl. No. 17/463,429.
Claims priority of provisional application 63/186,742, filed on May 10, 2021.
Claims priority of provisional application 63/072,898, filed on Aug. 31, 2020.
Prior Publication US 2022/0088180 A1, Mar. 24, 2022
Int. Cl. A61K 39/215 (2006.01); A61K 39/00 (2006.01); A61K 45/06 (2006.01); A61P 31/14 (2006.01); G01N 33/569 (2006.01); G01N 33/68 (2006.01)
CPC A61K 39/215 (2013.01) [A61K 45/06 (2013.01); A61P 31/14 (2018.01); G01N 33/56983 (2013.01); G01N 33/6818 (2013.01); A61K 2039/53 (2013.01); G01N 2333/165 (2013.01); G01N 2800/26 (2013.01)] 29 Claims
 
1. An immunogenic composition comprising:
one or more vectors each comprising one or more polynucleotides encoding one or more polypeptides, wherein the one or more polypeptides:
a. is capable of binding to Major Histocompatibility Complex (MHC) class I,
b. is derived from one or more proteins of SARS-COV-2 by expression of an internal out-of-frame open reading frame (ORF) of SARS-COV-2, wherein the internal out-of-frame ORF is S.iORF1 or S.iORF2, and
c. wherein at least one polypeptide of the one or more polypeptides derived from expression of S.iORF1 is MLLGSMLYM (SEQ ID NO: 4); or wherein at least one polypeptide of the one or more polypeptides is derived from expression of S.iORF1 or S.iORF2 is GPMVLRGLIT (SEQ ID NO: 6) or GLITLSYHL (SEQ ID NO: 5).