US 12,419,932 B2
Aqueous solution comprising a glutathione salt
Edward J. Delaney, Princeton, NJ (US); Carolyn Durham, Chapel Hill, NC (US); and Daniel W. Copeland, Chapel Hill, NC (US)
Assigned to Renovion, Inc., Chapel Hill, NC (US)
Filed by RENOVION, INC., Chapel Hill, NC (US)
Filed on Aug. 30, 2024, as Appl. No. 18/821,027.
Application 18/821,027 is a continuation of application No. 18/640,964, filed on Apr. 19, 2024, granted, now 12,097,238.
Application 18/640,964 is a continuation of application No. PCT/US2023/010081, filed on Jan. 4, 2023.
Claims priority of provisional application 63/296,405, filed on Jan. 4, 2022.
Prior Publication US 2024/0424052 A1, Dec. 26, 2024
This patent is subject to a terminal disclaimer.
Int. Cl. A61K 38/06 (2006.01); A61K 9/08 (2006.01); A61K 31/375 (2006.01)
CPC A61K 38/063 (2013.01) [A61K 9/08 (2013.01); A61K 31/375 (2013.01)] 3 Claims
 
1. A method of making a preparation comprising an aqueous solution in a closed container with a headspace, wherein:
(i) the aqueous solution comprises a salt having Formula I:

OG Complex Work Unit Chemistry
(ii) the atmosphere of the headspace comprises 90% or more carbon dioxide by volume; and
(iii) M+ is Na+,
the method comprising:
(i) dissolving L-glutathione, ascorbic acid, and sodium bicarbonate in water for injection under carbon dioxide to give an aqueous solution;
(ii) transferring a portion of the aqueous solution to a container;
(iii) overlaying the aqueous solution with carbon dioxide; and
(iv) sealing the container with a stopper,
wherein about 8 wt % to about 18 wt % of L-glutathione, about 3 wt % to about 13 wt % of ascorbic acid, and about 3 wt % to about 13 wt % of sodium bicarbonate is dissolved in about 62 wt % to about 82 wt % water for injection to give the aqueous solution.