US 12,419,570 B2
Thin, soft, skin-mounted microfluidic networks for detection and analysis of targets of interest in sweat
John A. Rogers, Wilmette, IL (US); Tyler R. Ray, Evanston, IL (US); Jungil Choi, Chicago, IL (US); and Yi Zhang, Evanston, IL (US)
Assigned to NORTHWESTERN UNIVERSITY, Evanston, IL (US)
Appl. No. 16/616,813
Filed by NORTHWESTERN UNIVERSITY, Evanston, IL (US)
PCT Filed Jun. 1, 2018, PCT No. PCT/US2018/035674
§ 371(c)(1), (2) Date Nov. 25, 2019,
PCT Pub. No. WO2018/223044, PCT Pub. Date Dec. 6, 2018.
Claims priority of provisional application 62/514,515, filed on Jun. 2, 2017.
Prior Publication US 2020/0093416 A1, Mar. 26, 2020
This patent is subject to a terminal disclaimer.
Int. Cl. A61B 5/00 (2006.01); A61B 5/01 (2006.01); A61B 5/103 (2006.01); A61B 5/145 (2006.01); A61B 5/1477 (2006.01); B01L 3/00 (2006.01); G01N 33/52 (2006.01); G01N 33/98 (2006.01)
CPC A61B 5/4266 (2013.01) [A61B 5/002 (2013.01); A61B 5/01 (2013.01); A61B 5/1032 (2013.01); A61B 5/14532 (2013.01); A61B 5/14539 (2013.01); A61B 5/14546 (2013.01); A61B 5/1477 (2013.01); A61B 5/4842 (2013.01); A61B 5/4845 (2013.01); A61B 5/4848 (2013.01); A61B 5/6832 (2013.01); B01L 3/502715 (2013.01); G01N 33/52 (2013.01); G01N 33/98 (2013.01); B01L 2200/16 (2013.01); B01L 2300/023 (2013.01); B01L 2300/027 (2013.01); B01L 2300/047 (2013.01); B01L 2300/0645 (2013.01); B01L 2300/0803 (2013.01); B01L 2300/123 (2013.01)] 29 Claims
OG exemplary drawing
 
1. A method for the detection of a biomarker in sweat comprising:
providing an epidermal microfluidic device to a skin of a subject, wherein conformal contact is established thereby providing fluidic communication between said epidermal microfluidic device and said skin, said epidermal microfluidic device comprising:
a flexible substrate comprising a first collection layer, a second collection layer and a capping layer disposed between the first collection layer and the second collection layer, wherein
the first collection layer contains a first channel network forming at least one first collection chamber having a sweat inlet to being opened said skin and an outlet to the second collection layer, wherein said sweat inlet is operably in the fluidic communication with said skin;
the second collection layer contains a second channel network forming a plurality of secondary collection chambers fluidically connected to the at least one first collection chamber, each of the secondary collection chambers having an inlet fluidically connected to said outlet of the at least one first collection chamber;
the capping layer is formed to seal both the first channel network of the first collection layer and the second channel network of the second collection layer; and
each of the first channel network and the second channel network is in a serpentine form; and
an analyzer integrated with or fluidically connected to the at least one first collection chamber and/or the plurality of secondary collection chambers and configured to analyze said sweat for one or more biomarkers;
collecting said sweat from said subject in said epidermal microfluidic device; and
identifying or quantifying the amount of said one or more biomarkers in said sweat.