US 12,419,340 B2
Microbial fermentation method for improving tobacco quality
Binbin Hu, Kunming (CN); Jing Mai, Kunming (CN); Mingjun Zhu, Kunming (CN); Congming Zou, Kunming (CN); Zhonglong Lin, Kunming (CN); Ying Ning, Kunming (CN); Yi Chen, Kunming (CN); Yonglei Jiang, Kunming (CN); Ling Zou, Kunming (CN); Guiyan Zhou, Kunming (CN); Baoxing Wang, Kunming (CN); Jieyun Cai, Kunming (CN); Haowei Sun, Kunming (CN); Ke Zhang, Kunming (CN); and Xiaowei Zhang, Kunming (CN)
Assigned to Yunnan Academy of Tobacco Agricultural Sciences, Kunming (CN)
Filed by Yunnan Academy of Tobacco Agricultural Sciences, Kunming (CN)
Filed on Sep. 26, 2022, as Appl. No. 17/952,611.
Claims priority of application No. 202210628009.1 (CN), filed on Jun. 6, 2022.
Prior Publication US 2023/0389594 A1, Dec. 7, 2023
Int. Cl. A24B 15/20 (2006.01); C12N 1/20 (2006.01); C12P 1/04 (2006.01)
CPC A24B 15/20 (2013.01) [C12N 1/20 (2013.01); C12P 1/04 (2013.01)] 9 Claims
OG exemplary drawing
 
1. A microbial fermentation method for improving tobacco quality, comprising the following steps:
(1) inoculating a flue-cured tobacco as an inoculation source into a tobacco powder medium and cultivating at 20-40° C. for 20-50 h to obtain a microbial liquid, wherein the tobacco powder medium comprises 8-12 g/L of tobacco powder, 8-12 g/L of tryptone, 4-6 g/L of yeast powder, and 4-6 g/L of sodium chloride;
(2) inoculating the microbial liquid in step (1) into the tobacco powder medium, cultivating at 20-40° C. for 20-50 h, and circulating for 15-25 cycles to obtain stable flue-cured tobacco microbial flora;
(3) inoculating the stable flue-cured tobacco microbial flora obtained in step (2) into the tobacco powder medium, and cultivating at 20-40° C. for 20-50 h to obtain seed liquid of the microbial flora;
(4) centrifuging the seed liquid obtained in step (3) and collecting microbes;
(5) washing the microbes collected in step (4);
(6) resuspending the washed microbes in step (5) to obtain a microbial suspension; and
(7) spraying the microbial suspension of step (6) on tobacco leaves for fermenting for 6-8 days.