US 12,419,327 B2
Method for preparing feed by bacteria-enzyme synergistic fermentation
Yu Deng, Wuxi (CN); Yin Mao, Wuxi (CN); and Guohui Li, Wuxi (CN)
Assigned to Jiangnan University, Wuxi (CN)
Filed by Jiangnan University, Wuxi (CN)
Filed on Feb. 25, 2022, as Appl. No. 17/680,480.
Application 17/680,480 is a continuation of application No. PCT/CN2020/114498, filed on Sep. 10, 2020.
Claims priority of application No. 201910943375.4 (CN), filed on Sep. 30, 2019; and application No. 202010278169.9 (CN), filed on Apr. 10, 2020.
Prior Publication US 2022/0174980 A1, Jun. 9, 2022
Int. Cl. A23K 10/12 (2016.01); A23K 10/14 (2016.01); C12N 1/20 (2006.01); C12N 9/42 (2006.01); C12N 9/50 (2006.01); C12P 1/04 (2006.01); C12R 1/25 (2006.01)
CPC A23K 10/12 (2016.05) [A23K 10/14 (2016.05); C12N 1/205 (2021.05); C12N 9/2437 (2013.01); C12N 9/50 (2013.01); C12P 1/04 (2013.01); A23V 2400/169 (2023.08); C12R 2001/25 (2021.05)] 3 Claims
OG exemplary drawing
 
1. A method for preparing a feed composition, comprising:
providing a raw material comprising: (a) camellia seed meal, and (b) enzymes, wherein the enzymes comprise alkaline protease and cellulase, to produce a mixture,
adding to the mixture an inoculum of Lactobacillus plantarum (L. plantarum) JUN-DY-6 collection number CCTCC M 2017138, at 1 to 5% to produce a fermentation mixture; and
fermenting the fermentation mixture to produce a feed composition,
wherein a moisture content of the raw material is 30 to 50%;
wherein the cellulase is present in the mixture at 300 to 400 U/g substrate;
wherein the alkaline protease is present in the mixture at 800 to 1200 U/g substrate; and
wherein the L. plantarum JUN-DY-6 is present at a concentration of greater than or equal to 106 CFU/g substrate.