	US 12,419,310 B2
	Methods and compositions for controlling root lesion nematodes
Natalie Breakfield, St. Louis, MO (US); and Gregg Bogosian, St. Louis, MO (US)
Assigned to NewLeaf Symbiotics, Inc., St. Louis, MO (US)
Filed by NewLeaf Symbiotics, Inc., St. Louis, MO (US)
Filed on Dec. 21, 2023, as Appl. No. 18/392,795.
Application 16/906,888 is a division of application No. 16/561,694, filed on Sep. 5, 2019, abandoned.
Application 16/561,694 is a division of application No. 15/373,203, filed on Dec. 8, 2016, granted, now 10,448,645, issued on Oct. 22, 2019.
Application 18/392,795 is a continuation of application No. 16/906,888, filed on Jun. 19, 2020, granted, now 11,871,753.
Claims priority of provisional application 62/266,115, filed on Dec. 11, 2015.
Prior Publication US 2024/0130374 A1, Apr. 25, 2024
Int. Cl. A01N 63/20 (2020.01); A01N 25/00 (2006.01)

CPC A01N 63/20 (2020.01)

23 Claims

1. A method for making a composition for treating a plant wherein said method comprises;

i) inoculating a liquid culture medium with Methylobacterium strain NLS0934 deposited as NRRL B-67341;

ii) growing the Methylobacterium strain in said medium to a titer of at least 5×10⁸CFU per ml; and

iii) harvesting the Methylobacterium from said medium.