US 12,419,282 B2
Non-human animals expressing humanized CD3 complex
Kara L. Olson, White Plains, NY (US); Eric Smith, New York, NY (US); Ka-Man Venus Lai, Seattle, WA (US); Andrew J. Murphy, Croton-on-Hudson, NY (US); Gavin Thurston, Millerton, NY (US); and Dayong Guo, Overland Park, KS (US)
Assigned to Regeneron Pharmaceuticals, Inc, Tarrytown, NY (US)
Filed by Regeneron Pharmaceuticals, Inc., Tarrytown, NY (US)
Filed on Feb. 15, 2024, as Appl. No. 18/442,444.
Application 18/442,444 is a division of application No. 17/118,241, filed on Dec. 10, 2020, granted, now 11,937,587.
Application 17/118,241 is a continuation of application No. 16/872,226, filed on May 11, 2020, granted, now 10,932,455, issued on Mar. 2, 2021.
Application 16/872,226 is a continuation of application No. 14/949,834, filed on Nov. 23, 2015, abandoned.
Claims priority of provisional application 62/106,999, filed on Jan. 23, 2015.
Claims priority of provisional application 62/083,653, filed on Nov. 24, 2014.
Prior Publication US 2024/0306616 A1, Sep. 19, 2024
This patent is subject to a terminal disclaimer.
Int. Cl. A01K 67/0278 (2024.01); A01K 67/0276 (2024.01); C07K 14/725 (2006.01); C12N 5/071 (2010.01); C12N 15/85 (2006.01); G01N 33/50 (2006.01)
CPC A01K 67/0278 (2013.01) [A01K 67/0276 (2013.01); C07K 14/7051 (2013.01); C12N 5/0602 (2013.01); C12N 15/8509 (2013.01); G01N 33/5088 (2013.01); A01K 2207/12 (2013.01); A01K 2207/15 (2013.01); A01K 2217/072 (2013.01); A01K 2217/075 (2013.01); A01K 2217/15 (2013.01); A01K 2227/105 (2013.01); A01K 2267/01 (2013.01); A01K 2267/03 (2013.01); A01K 2267/0337 (2013.01); Y02A 50/30 (2018.01)] 20 Claims
 
1. An isolated rodent cell comprising:
at an endogenous CD3ε locus, a nucleic acid sequence encoding a functional chimeric human/rodent CD3ε protein that comprises an extracellular domain of a human CD3ε protein,
at an endogenous CD3δ locus, a nucleic acid sequence encoding a functional chimeric human/rodent CD3δ protein that comprises an extracellular domain of a human CD3δ protein, and
at an endogenous CD3γ locus, a nucleic acid sequence encoding a functional chimeric human/rodent CD3γ protein that comprises an extracellular domain of a human CD3γ protein.