	US 12,419,236 B2
	Methods and compositions for improved plant regeneration from microspore-derived embryos
Ying Guo, Ballwin, MO (US); Daniel Jefferson Karcher, St. Louis, MO (US); Huachun Wang Larue, Chesterfield, MO (US); and Huai Wang, Chesterfield, MO (US)
Assigned to Monsanto Technology LLC, St. Louis, MO (US)
Filed by Monsanto Technology LLC, St. Louis, MO (US)
Filed on Nov. 29, 2023, as Appl. No. 18/523,087.
Claims priority of provisional application 63/430,998, filed on Dec. 7, 2022.
Prior Publication US 2024/0188502 A1, Jun. 13, 2024
Int. Cl. A01H 4/00 (2006.01); A01G 2/00 (2018.01); A01G 24/22 (2018.01)

CPC A01H 4/005 (2013.01) [A01G 2/00 (2018.02); A01G 24/22 (2018.02)]

23 Claims

1. A method of generating a plantlet, the method comprising:

(a) contacting a plurality of microspore-derived embryos with a liquid maturation medium at a concentration of about 5 to about 50 microspore-derived embryos per mL maturation medium for a time period sufficient to produce at least one matured microspore-derived embryo, wherein the maturation medium comprises a first plant hormone and about 430 mM to about 880 mM of a monosaccharide solute, a disaccharide solute, or a polysaccharide solute;

(b) transferring the matured microspore-derived embryo to a substrate; and