US 12,419,235 B2
Modified CULLIN1 gene
Lilian Van Der Linde, De Lier (NL); and Sara Movahedi, De Lier (NL)
Assigned to RIJK ZWAAN ZAADTEELT EN ZAADHANDEL B.V., De Lier (NL)
Filed by RIJK ZWAAN ZAADTEELT EN ZAADHANDEL B.V., De Lier (NL)
Filed on Mar. 10, 2020, as Appl. No. 16/813,919.
Application 16/813,919 is a continuation of application No. 15/892,500, filed on Feb. 9, 2018, granted, now 10,856,482.
Application 15/892,500 is a continuation in part of application No. PCT/EP2016/071177, filed on Sep. 8, 2016.
Claims priority of application No. 2015409 (NL), filed on Sep. 8, 2015.
Prior Publication US 2020/0196547 A1, Jun. 25, 2020
Int. Cl. A01H 1/00 (2006.01); C07K 14/415 (2006.01); C12N 15/82 (2006.01); C12Q 1/6895 (2018.01)
CPC A01H 1/121 (2021.01) [C07K 14/415 (2013.01); C12N 15/8261 (2013.01); C12Q 1/6895 (2013.01); Y02A 40/146 (2018.01)] 21 Claims
 
1. A method for selecting a Cucumis sativus plant, a Cucumis melo plant, a Cucurbita pepo plant, or a Citrullus lanatus plant comprising a modified Cullin1 gene and expressing a modified Cullin1 protein comprising a single non-silent amino acid substitution, wherein the Cucumis sativus plant, a Cucumis melo plant, a Cucurbita pepo plant, or a Citrullus lanatus plant expressing the modified Cullin1 protein exhibits a compact growth phenotype as compared to an isogenic line not comprising the modified Cullin 1 protein wherein the method comprises;
a) testing the plant for presence of the modified Cullin1 gene, or for the presence of the modified Cullin 1 protein,
wherein:
when the plant is a Cucumis sativus plant, the testing comprises detecting modification of the nucleotide sequence of SEQ ID NO: 1 leading to change of the amino acid sequence of SEQ ID NO: 18, or detecting modification of the amino acid sequence of SEQ ID NO: 18 in the region that binds SKP1 and/or ETA2, wherein the modification of the nucleotide sequence of SEQ ID NO: 1 confers a substitution in the Cullin1polypeptide sequence of SEQ ID NO: 18 between positions 30 to 60 of SEQ ID NO: 18, wherein the modification of the nucleotide sequence excludes the substitution of A to G at position 147 of SEQ ID NO: 1;
when the plant is a Cucumis melo plant, the testing comprises detecting modification of the nucleotide sequence of SEQ ID NO: 2 leading to change of the amino acid sequence of SEQ ID NO: 19, or detecting modification of the amino acid sequence of SEQ ID NO: 19 in the region that binds SKP1 and/or ETA2 wherein the modification of the nucleotide sequence of SEQ ID NO: 2 confers a substitution in the Cullin1polypeptide sequence of SEQ ID NO: 19 between positions 30 to 60 of SEQ ID NO: 19;
when the plant is a Cucurbita pepo plant, the testing comprises detecting modification of the nucleotide sequence of SEQ ID NO: 3 leading to change of the amino acid sequence of SEQ ID NO: 20, or detecting modification of the amino acid sequence of SEQ ID NO: 20 in the region that binds SKP1 and/or ETA2 wherein the modification of the nucleotide sequence of SEQ ID NO: 3 confers a substitution in the Cullin 1polypeptide sequence of SEQ ID NO: 20 between positions 30 to 60 of SEQ ID NO: 20;
when the plant is a Citrullus lanatus plant, the testing comprises detecting modification of the nucleotide sequence of SEQ ID NO: 4 leading to change of the amino acid sequence of SEQ ID NO: 21, or detecting modification of the amino acid sequence of SEQ ID NO: 21 in the region that binds SKP1 and/or ETA2 wherein the modification of the nucleotide sequence of SEQ ID NO: 4 confers a substitution in the Cullin 1polypeptide sequence of SEQ ID NO: 21 between positions 30 to 60 of SEQ ID NO: 21;
b) selecting the Cucumis sativus plant, Cucumis melo plant, Cucurbita pepo plant, or Citrullus lanatus plant having the modified Cullin1 gene or the modified Cullin 1 protein; and
c.) growing the Cucumis sativus plant, Cucumis melo plant, Cucurbita pepo plant,
or Citrullus lanatus plant exhibiting a compact growth phenotype compared to an isogenic line not comprising the modified Cullin1 gene.