US 12,092,645 B2
Method for discriminating a microorganism
Hiroto Tamura, Nagoya (JP); Naomi Yamamoto, Nagoya (JP); Teruyo Kato, Toyota (JP); Keisuke Shima, Kyoto (JP); and Shinji Funatsu, Kyoto (JP)
Assigned to SHIMADZU CORPORATION, Kyoto (JP); and MEIJO UNIVERSITY, Nagoya (JP)
Filed by SHIMADZU CORPORATION, Kyoto (JP); and MEIJO UNIVERSITY, Nagoya (JP)
Filed on Nov. 28, 2022, as Appl. No. 18/059,404.
Application 18/059,404 is a continuation of application No. 16/089,922, granted, now 11,561,228, previously published as PCT/JP2016/060868, filed on Mar. 31, 2016.
Prior Publication US 2023/0213528 A1, Jul. 6, 2023
This patent is subject to a terminal disclaimer.
Int. Cl. G01N 33/68 (2006.01); C12Q 1/04 (2006.01); C12N 15/09 (2006.01)
CPC G01N 33/6848 (2013.01) [C12Q 1/04 (2013.01); C12N 15/09 (2013.01); G01N 2333/195 (2013.01); G01N 2560/00 (2013.01)] 2 Claims
OG exemplary drawing
 
1. A method for discriminating a microorganism, the method comprising:
a) a step of subjecting a sample containing a microorganism to mass spectrometry to obtain a mass spectrum;
b) a reading step of reading a mass-to-charge ratio m/z of a peak derived from a marker protein from the mass spectrum; and
c) a discrimination step of discriminating which bacterial species of Listeria bacteria is contained in the sample based on the mass-to-charge ratio m/z,
wherein
(i) at least one of ribosomal proteins L24, L6, L18, L15, S11, S9, and S16, and (ii) L31 are used as the marker protein; and
a bacterial species of the Listeria bacteria is one of Listeria innocua, Listeria welshimeri, Listeria seeligeri, Listeria ivanovii, Listeria grayi, and Listeria rocourtiae.