	US 12,092,644 B2
	Kits comprising aldehydes to reduce artifacts in analyzing glycans released from glycoproteins or glycopeptides
Michael J Kimzey, Laguna Hills, CA (US)
Assigned to Agilent Technologies, Inc., Santa Clara, CA (US)
Filed by Agilent Technologies, Inc., Santa Clara, CA (US)
Filed on May 13, 2021, as Appl. No. 17/319,868.
Application 17/319,868 is a division of application No. 16/322,900, granted, now 11,035,862, previously published as PCT/US2017/047229, filed on Aug. 16, 2017.
Claims priority of provisional application 62/376,344, filed on Aug. 17, 2016.
Prior Publication US 2021/0263043 A1, Aug. 26, 2021
Int. Cl. G01N 33/58 (2006.01); C09B 19/00 (2006.01); C09B 57/02 (2006.01); C09B 57/08 (2006.01); C12P 19/04 (2006.01); G01N 33/53 (2006.01); G01N 33/68 (2006.01)

CPC G01N 33/6848 (2013.01) [C09B 19/00 (2013.01); C09B 57/02 (2013.01); C09B 57/08 (2013.01); C12P 19/04 (2013.01); G01N 33/5308 (2013.01); G01N 33/582 (2013.01); G01N 33/6854 (2013.01); G01N 2400/10 (2013.01)]

14 Claims

1. A glycosylamines labeling kit, said kit comprising,

(a) an aldehyde selected from the group consisting of DL-glyceraldehyde, glyceraldehyde dimer, glycoaldehyde dimer, erythrose, pyruvaldehyde, 2-ethylbutyraldehyde, 2-methylbutyraldehyde, 2-methylvaleraldehyde, 4-methyl-4-nitrovaleraldehyde, acetaldehyde, butyraldehyde, isobutyraldehyde, propionaldehyde, or valeraldehyde,

(b) an amine-reactive dye capable of covalently binding to a free amino or amine end group of a glycoprotein or a glycopeptide under conditions allowing said amine reactive dye to covalently bind to said free amino or amine end group of said glycoprotein or said glycopeptide, and