US 12,092,641 B2
Chemical detection assays
Richard C. Willson, Houston, TX (US); Victoria M. Hlavinka, Houston, TX (US); Binh V. Vu, Houston, TX (US); Federico Augusto Ruiz-Ruiz, Houston, TX (US); Marco Antonio Rito Palomares, Houston, TX (US); and Mary A. Crum, Houston, TX (US)
Appl. No. 17/271,077
Filed by Richard C. Willson, Houston, TX (US); Victoria M. Hlavinka, Houston, TX (US); Binh V. Vu, Houston, TX (US); Federico Augusto Ruiz-Ruiz, Houston, TX (US); Marco Antonio Rito Palomares, Houston, TX (US); and Mary A. Crum, Houston, TX (US)
PCT Filed Aug. 22, 2019, PCT No. PCT/US2019/047708
§ 371(c)(1), (2) Date Feb. 24, 2021,
PCT Pub. No. WO2020/041591, PCT Pub. Date Feb. 27, 2020.
Claims priority of provisional application 62/722,409, filed on Aug. 24, 2018.
Prior Publication US 2022/0057406 A1, Feb. 24, 2022
Int. Cl. B01L 3/00 (2006.01); B01F 23/00 (2022.01); B01F 23/41 (2022.01); B01F 101/23 (2022.01); B23Q 17/24 (2006.01); C12M 1/34 (2006.01); C12Q 1/04 (2006.01); C12Q 1/18 (2006.01); C12Q 1/32 (2006.01); C12Q 1/54 (2006.01); C12Q 1/686 (2018.01); G01N 21/3577 (2014.01); G01N 21/359 (2014.01); G01N 21/39 (2006.01); G01N 21/45 (2006.01); G01N 21/64 (2006.01); G01N 21/76 (2006.01); G01N 30/12 (2006.01); G01N 30/68 (2006.01); G01N 30/70 (2006.01); G01N 30/72 (2006.01); G01N 30/88 (2006.01); G01N 33/00 (2006.01); G01N 33/18 (2006.01); G01N 33/50 (2006.01); G01N 33/66 (2006.01); G01N 33/68 (2006.01); G01N 33/72 (2006.01)
CPC G01N 33/66 (2013.01) [C12Q 1/32 (2013.01); C12Q 1/54 (2013.01); G01N 21/763 (2013.01); G01N 33/723 (2013.01)] 17 Claims
 
1. A method for analyzing a sample, the method comprising:
(a) obtaining the sample comprising 1,5-anhydroglucitol and optionally a first species;
(c) adding a first reagent to the sample, wherein the first reagent causes a first chemiluminescent or bioluminescent reaction with 1,5-anhydroglucitol in the sample; and
(d) measuring a first light response resulting from the first chemiluminescent or bioluminescent reaction;
(e) adding a second reagent to the sample, wherein:
(i) the second reagent is added before or after the first reagent to the sample; and
(ii) the second reagent causes a second chemiluminescent reaction with the first species in the sample;
(f) measuring a second light response resulting from the second chemiluminescent reaction; and
(g) comparing the first light response to the second light response to determine a ratio of 1,5-anhydroglucitol and the first species.