US 12,092,601 B2
Construction method for photocathode indirect competition sensor and evaluation method
Zhaowei Zhang, Wuhan (CN); Wenqin Wu, Wuhan (CN); Xiao Chen, Wuhan (CN); Ling Cheng, Wuhan (CN); Li Yu, Wuhan (CN); Xiupin Wang, Wuhan (CN); and Peiwu Li, Wuhan (CN)
Assigned to Oil Crops Research Institute of Chinese Academy of Agricultural Sciences, Wuhan (CN); and Wuhan Norrida Biotechnological Co., LTD, Wuhan (CN)
Filed by Oil Crops Research Institute of Chinese Academy of Agricultural Sciences, Wuhan (CN); and Wuhan Norrida Biotechnological Co., LTD, Wuhan (CN)
Filed on Jun. 28, 2023, as Appl. No. 18/342,808.
Claims priority of application No. 202210747865.9 (CN), filed on Jun. 29, 2022.
Prior Publication US 2023/0341346 A1, Oct. 26, 2023
Int. Cl. G01N 27/30 (2006.01); G01N 21/31 (2006.01); G01N 23/20 (2018.01); G01N 23/20091 (2018.01); G01N 23/2251 (2018.01); G01N 24/00 (2006.01); G01N 27/27 (2006.01); G01N 27/327 (2006.01); G01N 33/569 (2006.01)
CPC G01N 27/305 (2013.01) [G01N 21/31 (2013.01); G01N 23/20 (2013.01); G01N 23/20091 (2013.01); G01N 23/2251 (2013.01); G01N 24/00 (2013.01); G01N 27/27 (2013.01); G01N 27/3275 (2013.01); G01N 33/56961 (2013.01); G01N 2333/38 (2013.01)] 6 Claims
 
1. A construction method for a photocathode indirect competition sensor, comprising the following steps:
S1), synthesizing Bi2O3/CuBi2O4, using a high-temperature calcination method, uniformly dispersing Bi2O3/CuBi2O4 in a dispersion solution by ultrasound, and drop coating Indium Tin Oxide (ITO) conductive glass, and drying the ITO conductive glass to obtain a Bi2O3/CuBi2O4/ITO-based sensing platform;
wherein S1) comprises:
grinding Cu(NO3)2·3H2O, Bi(NO3)3·5H2O, and glucose at a molar ratio of 1:1:7 to 1:5:7 in a quartz agate mortar for 5-10 minutes to obtain a mixture;
drying the mixture at 60° C. in a ceramic crucible to obtain an anhydrous precursor of Bi2O3/CuBi2O4;
heating the anhydrous precursor to 400° C., and maintaining the temperature in a tube furnace for 20-40 minutes to obtain combustion residues; and
grinding the combustion residues in the quartz agate mortar, then calcining at 500° C. for 2-6 hours in the ceramic crucible, and finally obtaining Bi2O3/CuBi2O4; and
S2), obtaining the photocathode indirect competition sensor based on the Bi2O3/CuBi2O4/ITO-based sensing platform;
wherein the S2) specifically comprises:
dripping 3-10 μL of glutaraldehyde (GLD) aqueous solution onto the Bi2O3/CuBi2O4/ITO-based sensing platform, incubating at a room temperature for 30-60 minutes, performing rinsing with 0.1 M phosphate buffer solution (PBS) to remove unconjugated glutaraldehyde molecules, and obtaining GLD/Bi2O3/CuBi2O4/ITO;
performing drop coating on the GLD/Bi2O3/CuBi2O4/ITO with a target antigen solution with a predefined concentration, incubating at 4° C., and performing rinsing with the 0.1 M PBS to obtain Ag/GLD/Bi2O3/CuBi2O4/ITO;
dropwise adding a 1% bovine serum albumin (BSA) solution onto the Ag/GLD/Bi2O3/CuBi2O4/ITO, incubating at a room temperature, performing rinsing with the 0.1 M PBS to obtain BSA/Ag/GLD/Bi2O3/CuBi2O4/ITO as the photocathode indirect competition sensor.