US 12,092,561 B2
Reagent, method for analyzing platelets and blood cell analyzer
Gengwen Chen, Shenzhen (CN); Ziqian Zhang, Shenzhen (CN); Yi Ye, Shenzhen (CN); and Zhaoyang Li, Shenzhen (CN)
Assigned to Shenzhen Mindray Bio-Medical Electronics Co., Ltd., Shenzhen (CN)
Filed by Shenzhen Mindray Bio-Medical Electronics Co., Ltd., Shenzhen (CN)
Filed on Oct. 27, 2020, as Appl. No. 17/081,712.
Application 17/081,712 is a continuation of application No. PCT/CN2019/084648, filed on Apr. 26, 2019.
Claims priority of application No. 201810402750.X (CN), filed on Apr. 28, 2018.
Prior Publication US 2021/0041341 A1, Feb. 11, 2021
Int. Cl. G01N 15/00 (2024.01); G01N 15/14 (2006.01); G01N 33/50 (2006.01); G01N 15/01 (2024.01)
CPC G01N 15/147 (2013.01) [G01N 33/5094 (2013.01); G01N 2015/011 (2024.01); G01N 2015/012 (2024.01); G01N 2015/018 (2024.01)] 22 Claims
OG exemplary drawing
 
1. A method for analyzing a blood sample, comprising:
treating the blood sample with a first reagent and a second reagent to prepare a test sample, wherein the first reagent is a hemolytic agent that hemolyzes red blood cells in the test sample, and the second reagent is a membrane-specific dye or a mitochondrion-specific dye that stains both platelets and white blood cells in the test sample;
passing particles in the test sample through a detection area of an optical detection system one by one to obtain scattered light intensity information and fluorescence intensity information of each particle in the test sample;
differentiating, in a single test channel, the platelets and the white blood cells in the test sample according to the scattered light intensity information and the fluorescence intensity information of each particle in the test sample, wherein the staining by the membrane-specific dye or the mitochondrion-specific dye differentiates the platelets from fragments of the red blood cells produced by the hemolytic agent, the membrane-specific dye is a fluorescence dye capable of specifically staining cell membranes, and the mitochondrion-specific dye is a fluorescence dye capable of specifically staining cell mitochondria; and
counting the platelets and classifying the white blood cells according to the scattered light intensity information and the fluorescence intensity information of each particle in the test sample.