US 12,090,227 B2
Method to improve the encapsulation efficiency and physicochemical stability of ginsenosides Rg3 and CK nano-emulsion
Peng Zhou, Wuxi (CN); Changshu Liu, Suzhou (CN); Yaowei Liu, Wuxi (CN); Tao Yang, Wuxi (CN); Yan Zheng, Wuxi (CN); Jianguo Liu, Suzhou (CN); Kexin Li, Shanghai (CN); and Shuyan Lu, Shanghai (CN)
Assigned to JIANGNAN UNIVERSITY, Wuxi (CN); STANDARD FOODS (CHINA) CO., LTD, Suzhou (CN); and LE BONTA WELLNESS CO., LTD, Shanghai (CN)
Filed by Jiangnan University, Wuxi (CN); Standard Foods (China) Co., Ltd, Suzhou (CN); and Le Bonta Wellness Co., Ltd, Shanghai (CN)
Filed on Dec. 1, 2023, as Appl. No. 18/526,168.
Application 18/526,168 is a continuation of application No. PCT/CN2023/101753, filed on Jun. 21, 2023.
Claims priority of application No. 202210867114.0 (CN), filed on Jul. 22, 2022.
Prior Publication US 2024/0099972 A1, Mar. 28, 2024
Int. Cl. A61K 9/00 (2006.01); A61K 9/107 (2006.01); A61K 31/7028 (2006.01); A61K 47/26 (2006.01); A61K 47/42 (2017.01); A61K 47/44 (2017.01)
CPC A61K 9/1075 (2013.01) [A61K 31/7028 (2013.01); A61K 47/26 (2013.01); A61K 47/42 (2013.01); A61K 47/44 (2013.01)] 7 Claims
 
1. A method to improve the encapsulation efficiency and physicochemical stability of ginsenosides Rg3 and CK nano-emulsion, which comprises:
obtaining an aqueous phase comprising whey protein isolate (WPI), water, and Tween 80, by:
dissolving WPI in water, heating the WPI in water at 80 to 90° C. for 15 to 20 minutes,
cooling the WPI in water to room temperature (25±2° C.) with an ice water bath,
adding Tween 80 to the WPI and water, stirring the WPI, water and Tween 80 at 200 to 400 rpm for 2 to 4 hours, and
mixing the WPI, Tween 80 and water,
resting the WPI, Tween 80, and water at 4° C. for 10 to 12 hours until the WPI, Tween 80, and water is completely hydrated to obtain the aqueous phase;
wherein the ratio of WPI, Tween 80, and water in the aqueous phase is 0.5 g:0.5 g to 0.7 g:50 mL,
obtaining an oil phase comprising a saponin extract with ginsenosides Rg3 and CK and oil by preparing the saponin extract with ginsenosides Rg3 and CK by:
mixing ginseng tablets with a citric acid solution with a pH value of 1 to 7,
heating the ginseng tablets and citric acid mixture at 130° C. for 1 to 5 hours to obtain the saponin extract containing the minor ginsenosides Rg3 and CK;
mixing the saponin extract containing minor ginsenosides Rg3 and CK with an edible oil selected from the group consisting of: rapeseed oil, soybean oil, sunflower seed oil, and peanut oil, to obtain the oil phase; and
obtaining an oil-in-water nano-emulsion that comprises the minor ginsenosides Rg3 and CK by:
mixing the aqueous phase with the oil phase at a volume ratio of the aqueous phase to the oil phase of 1:1,
performing shearing dispersion of the aqueous phase and oil phase mixture to obtain a coarse emulsion,
subjecting the coarse emulsion to microfluidization homogenization to obtain the oil-in-water nano-emulsion containing the minor ginsenosides Rg3 and CK.