US 12,089,959 B2
High resolution intravital imaging and uses thereof
David Entenberg, Granite Springs, NY (US); John S. Condeelis, Bronx, NY (US); and Sonia E. Voiculescu, Bronx, NY (US)
Assigned to Albert Einstein College of Medicine, Bronx, NY (US)
Filed by Albert Einstein College of Medicine, Bronx, NY (US)
Filed on Jun. 21, 2023, as Appl. No. 18/339,020.
Application 18/339,020 is a continuation of application No. 16/640,869, granted, now 11,712,205, previously published as PCT/US2018/044737, filed on Aug. 1, 2018.
Claims priority of provisional application 62/548,455, filed on Aug. 22, 2017.
Prior Publication US 2024/0000391 A1, Jan. 4, 2024
Int. Cl. A61B 5/00 (2006.01); A61B 5/08 (2006.01); G02B 21/36 (2006.01)
CPC A61B 5/704 (2013.01) [A61B 5/0068 (2013.01); A61B 5/0071 (2013.01); A61B 5/08 (2013.01); A61B 5/418 (2013.01); A61B 5/4222 (2013.01); A61B 5/4872 (2013.01); G02B 21/367 (2013.01); A61B 2503/42 (2013.01)] 5 Claims
OG exemplary drawing
 
1. A method for chronic intravital imaging of the lung of a subject comprising making a circular incision through the skin and adjacent ribs of an anesthetized subject lateral to the sternum and superior to the last floating rib, securing a window frame in the incision so that the adjacent ribs are positioned in a groove of the window frame, wherein the window frame comprises fiducial marks, adhering the window frame to lung tissue by raising the window frame away from the lung tissue, dispensing a quick-setting adhesive on the underside of the window frame, and expanding the lung to make contact with the window frame until the adhesive sets, suturing tissue around the window frame to provide a complete surgical closure, sealing the thoracic cavity by applying an adhesive to a window and adhering it to the expanded lung tissue and the inner recess of the window frame, and following recovery of the subject from anesthesia, stabilizing motion of the window frame for imaging by placing a fixturing plate, comprising portions defining a slotted recess sized to accommodate the window frame, between skin of the subject and an outside lip of the window frame, and placing the subject on the stage of a microscope
acquiring through the window, images of the lung tissue to produce an overview image of the lung tissue that preserves subcellular resolution.