| CPC A01K 63/02 (2013.01) [A01K 63/04 (2013.01)] | 1 Claim |
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1. A transport method for fish using a keeping-alive box, the keeping-alive box comprising:
a box body; and
a cover assembly;
wherein a top of the box body is provided with an opening; and the cover assembly is arranged at the opening to allow opening or closing of the box body;
the box body comprises a fish accommodating area and a water circulation area; and the water circulation area is arranged at a side inside the box body;
the cover assembly comprises a first cover and a second cover; the first cover is arranged at the opening corresponding to the fish accommodating area, and is pivotedly connected to the box body; and the first cover is provided with a vent hole to prevent splash;
the second cover is arranged at the opening corresponding to the water circulation area;
a connection between the cover assembly and the box body is provided with a splash-proof rubber;
the water circulation area comprises a purification system, a refrigerator and an aeration system; part of the aeration system is arranged in the water circulation area, and the other part of the aeration system is arranged in the fish accommodating area; the fish accommodating area is communicated with the purification system; the purification system is communicated with the refrigerator; the refrigerator is communicated with the fish accommodating area, such that water in the fish accommodating area is allowed to sequentially pass through the purification system and the refrigerator, and then flow back to the fish accommodating area; the purification system is configured to purify water passing from the fish accommodating area to the water circulation area; the refrigerator is configured to cool the water passing from the fish accommodating area to the water circulation area to a desired temperature and maintain it; and the aeration system is configured to maintain a dissolved oxygen in the water in the fish accommodating area at a desired level;
the purification system comprises a primary filtration area, a secondary filtration area, an activated carbon filtration area and an ultraviolet (UV) sterilization area arranged sequentially; the primary filtration area is communicated with the fish accommodating area; the UV sterilization area is communicated with the refrigerator; and the water in the fish accommodating area is allowed to sequentially flow through the primary filtration area, the secondary filtration area, the activated carbon filtration area and the UV sterilization area to enter the refrigerator;
the refrigerator is provided with a water inlet and a water outlet; the water inlet of the refrigerator is communicated with the UV sterilization area; and the water outlet of the refrigerator is communicated with the fish accommodating area; and
the aeration system comprises an aeration device and an aerator; the aeration device is hermetically connected to the aerator; the aerator is located in the water circulation area; the aeration device is located in the fish accommodating area; the aerator is configured to supply power to the aeration device; the aeration device is configured to perform aeration in the water of the fish accommodating area to maintain the dissolved oxygen in the fish accommodating area at the desired level; the water circulation area further comprises a dry area inside; the refrigerator and the aerator are located in the dry area; and the dry area is communicated with the outside through an air discharge hole;
the method comprising:
(S1) 36 h before transportation, subjecting fish to temporary culture in water with a temperature of 20-22° C., a salinity of 16%o, a dissolved oxygen level of 4-6 mg/L, and a pH of 7.5-8.5, wherein a volume ratio of the fish to the water is 1:50;
(S2) adding an emulsified Melissa officinalis essential oil into water in the keeping-alive box, wherein the emulsified Melissa officinalis essential oil is prepared through a step of:
evenly mixing Melissa officinalis essential oil with a concentration of 40 mg/L, ethyl alcohol with a volume fraction of 50% and polysorbate 80 in a volume ratio of 10:200:1;
(S3) transferring the fish to the keeping-alive box, wherein a volume ratio of the fish to the water in the keeping-alive box is 1:4; and cooling, by the refrigerator, the water in the keeping-alive box from 20-22° C. to 12° C. at a rate of 3° C./h;
(S4) covering the box body with the cover assembly followed by transportation for 72 h; wherein during the transportation, a water temperature is controlled to 12° C.±1° C.; and
(S5) transferring the fish to clean water with a temperature of 12° C.±1° C. followed by restoration to room temperature.
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