	US 12,089,547 B2
	Endophyte screening
Wayne Roydon Simpson, Palmerston North (NZ); Richard David Johnson, Palmerston North (NZ); and Hisashi Tsujimoto, Tottori (JP)
Assigned to Grasslanz Technology Limited, Lincoln (NZ); National University Corporation Tottori University, Tottori (JP); and The Grains Research and Development Corporation, Barton (AU)
Filed by National University Corporation Tottori University, Tottori (JP); Grasslanz Technology Limited, Lincoln (NZ); and The Grains Research and Development Corporation, Barton (AU)
Filed on Feb. 15, 2023, as Appl. No. 18/110,056.
Application 18/110,056 is a continuation of application No. 16/971,647, abandoned, previously published as PCT/IB2019/051395, filed on Feb. 20, 2019.
Claims priority of application No. 740055 (NZ), filed on Feb. 21, 2018.
Prior Publication US 2023/0320310 A1, Oct. 12, 2023
Int. Cl. A01H 17/00 (2006.01); A01N 63/30 (2020.01); C12Q 1/6895 (2018.01)

CPC A01H 17/00 (2013.01) [A01N 63/30 (2020.01); C12Q 1/6895 (2013.01); C12Q 2600/156 (2013.01)]

13 Claims

1. A method of identifying a wheat plant that forms a stable symbiotic combination with a fungal endophyte comprising

artificially inoculating a fungal endophyte into a candidate wheat plant to form a wheat plant/endophyte combination,

propagating the wheat plant/endophyte combination,

obtaining seed from the propagated combination, and

identifying the presence of the endophyte in the seed, and

selecting the wheat plant that is capable of forming a stable symbiotic combination with the fungal endophyte, wherein the fungal endophyte is an isolated strain of Epichloë fungal endophyte selected from the group consisting of AR3002 (NRRL #50579), AR3013 (NRRL #67557), AR3067 (NRRL #50719), AR3070 (NRRL #67564), and AR3108 (NRRL #67572).