	US 12,416,639 B2
	Reagents and methods for detecting protein lysine lactylation
Yingming Zhao, Chicago, IL (US); Di Zhang, Hangzhou (CN); and Lunzhi Dai, Chicago, IL (US)
Assigned to Jingjie PTM Biolab (Hangzhou) Co., Inc., Hangzhou (CN)
Appl. No. 17/416,955
Filed by Jingjie PTM Biolab (Hangzhou) Co., Inc, Hangzhou (CN)
PCT Filed Dec. 19, 2019, PCT No. PCT/US2019/067509 § 371(c)(1), (2) Date Jun. 21, 2021, PCT Pub. No. WO2020/132260, PCT Pub. Date Jun. 25, 2020.
Claims priority of provisional application 62/783,926, filed on Dec. 21, 2018.
Prior Publication US 2022/0091129 A1, Mar. 24, 2022
Int. Cl. G01N 33/68 (2006.01)

CPC G01N 33/6812 (2013.01)

6 Claims

1. A method for detecting a lactylated lysine in a protein or a fragment thereof, comprising:

(a) contacting the protein or a fragment thereof with an affinity reagent selected from the group consisting of pan anti-Kla (PTM-1401) antibody, anti-H3K18la (PTM-1406) antibody, and anti-H4K5la (PTM-1407) antibody, whereby a binding complex of the protein or a fragment thereof and the affinity reagent is formed, and

(b) detecting the binding complex, wherein the presence of the binding complex indicates the presence of a lactylated lysine in the protein or a fragment thereof.