US 12,416,624 B2
Faradaic systems and methods for self-limiting protein pore insertion in amembrane
Geoffrey Barrall, San Diego, CA (US); Eric Takeshi Harada, San Jose, CA (US); Jason David Komadina, Livermore, CA (US); J. William Maney, Jr., Emerald Hills, CA (US); and Charlotte Yang, Vancouver, WA (US)
Assigned to Roche Sequencing Solutions, Inc., Pleasanton, CA (US)
Filed by Roche Sequencing Solutions, Inc., Pleasanton, CA (US)
Filed on Dec. 9, 2022, as Appl. No. 18/064,037.
Application 18/064,037 is a continuation of application No. PCT/EP2021/065252, filed on Jun. 8, 2021.
Claims priority of provisional application 62/705,097, filed on Jun. 10, 2020.
Prior Publication US 2023/0105456 A1, Apr. 6, 2023
Int. Cl. G01N 33/487 (2006.01); C12Q 1/6869 (2018.01)
CPC G01N 33/48721 (2013.01) [C12Q 1/6869 (2013.01); C12Q 2565/607 (2013.01); C12Q 2565/631 (2013.01)] 31 Claims
OG exemplary drawing
 
1. A method of forming an array of nanopore sensor cells, the method comprising:
introducing a nanopore proximate to a cell in a solution comprising a first species of a redox couple but not a second species of the redox couple, the cell having a working electrode and a membrane sealing the cell, wherein the working electrode is powered by an electrically coupled power source;
applying a first voltage waveform across the membrane of the cell, wherein the voltage waveform starts at first voltage and increases in magnitude over a period of time to a second voltage, wherein the first voltage waveform has a polarity that maintains the first species of the redox couple in its current oxidation state;
inserting the nanopore into the membrane during the step of applying the first voltage waveform; and
applying a second voltage waveform having a polarity that oxidizes or reduces the first species to the second species.