	US 12,416,567 B2
	Flow cells with patterned bonding regions
Casey Scott Irvin, San Marcos, CA (US); Jonathan Ziebarth, Solana Beach, CA (US); Michael Rapp, La Jolla, CA (US); Danny Yuan Chan, San Diego, CA (US); Innsu Daniel Kim, San Diego, CA (US); and Avishek Aiyar, San Diego, CA (US)
Assigned to Illumina, Inc., San Diego, CA (US)
Filed by ILLUMINA, INC., San Diego, CA (US)
Filed on Aug. 24, 2023, as Appl. No. 18/455,467.
Claims priority of provisional application 63/373,617, filed on Aug. 26, 2022.
Prior Publication US 2024/0102913 A1, Mar. 28, 2024
Int. Cl. G01N 15/1434 (2024.01)

CPC G01N 15/1436 (2013.01)

25 Claims

1. A flow cell, comprising:

a patterned substrate having an active region and a bonding region that at least partially surrounds the active region, wherein the active region includes:

first depressions defined in a layer of the patterned substrate;

surface chemistry positioned in the first depressions; and

first interstitial regions surrounding the first depressions;

and wherein the bonding region includes:

second depressions defined in the layer; and

second interstitial regions surrounding the second depressions;

an adhesive positioned in the second depressions and over the second interstitial regions; and

a cover attached to the adhesive such that a flow channel is defined between a portion of the cover and the active region.