US 12,416,102 B2
Systems and methods for transfer of reagents between droplets
Solongo B. Ziraldo, Pleasanton, CA (US); Geoffrey McDermott, Livermore, CA (US); and Shea Thompson Lance, Livermore, CA (US)
Assigned to 10X GENOMICS, INC., Pleasanton, CA (US)
Filed by 10X Genomics, Inc., Pleasanton, CA (US)
Filed on Aug. 9, 2021, as Appl. No. 17/397,775.
Application 17/397,775 is a continuation of application No. PCT/US2020/017785, filed on Feb. 11, 2020.
Claims priority of provisional application 62/934,256, filed on Nov. 12, 2019.
Claims priority of provisional application 62/804,633, filed on Feb. 12, 2019.
Prior Publication US 2022/0025438 A1, Jan. 27, 2022
Int. Cl. C40B 50/06 (2006.01)
CPC C40B 50/06 (2013.01) 21 Claims
OG exemplary drawing
 
1. A method for nucleic acid processing, comprising:
(a) generating a plurality of droplets by agitating:
(i) a first fluid volume comprising a population of beads and a population of analyte carriers, wherein a bead of the population of beads comprises a plurality of nucleic acid barcode molecules, and wherein an analyte carrier of the population of analyte carriers comprises a plurality of nucleic acid molecules, and
(ii) a second fluid volume immiscible with the first fluid volume,
wherein a first droplet of the plurality of droplets comprises the bead of the population of beads and the analyte carrier of the population of analyte carriers;
(b) subjecting the first droplet and a second droplet comprising a reagent to conditions sufficient to transfer the reagent to the first droplet from the second droplet via a mediator comprising an aggregate of surfactant molecules and the reagent, wherein the reagent is configured to release the plurality of nucleic acid molecules from the analyte carrier; and
(c) generating a barcoded nucleic acid molecule using a nucleic acid barcode molecule of the plurality of nucleic acid barcode molecules and a nucleic acid molecule of the plurality of nucleic acid molecules.