US 12,416,033 B2
Assays and other reactions involving droplets
David A. Weitz, Cambridge, MA (US); Jeremy Agresti, Cambridge, MA (US); Liang-Yin Chu, Cambridge, MA (US); Jin-Woong Kim, Cambridge, MA (US); Amy Rowat, Cambridge, MA (US); Morten Sommer, Cambridge, MA (US); Gautam Dantas, Cambridge, MA (US); and George M. Church, Cambridge, MA (US)
Assigned to President and Fellows of Harvard College, Cambridge, MA (US)
Filed by President and Fellows of Harvard College, Cambridge, MA (US)
Filed on Sep. 20, 2024, as Appl. No. 18/892,086.
Application 18/892,086 is a continuation of application No. 17/148,796, filed on Jan. 14, 2021.
Application 17/148,796 is a continuation of application No. 16/779,501, filed on Jan. 31, 2020, granted, now 10,941,430.
Application 16/779,501 is a continuation of application No. 16/431,354, filed on Jun. 4, 2019, granted, now 10,738,337.
Application 16/431,354 is a continuation of application No. 15/792,218, filed on Oct. 24, 2017, granted, now 10,508,294.
Application 15/792,218 is a continuation of application No. 14/721,558, filed on May 26, 2015, granted, now 9,850,526.
Application 14/721,558 is a continuation of application No. 14/172,326, filed on Feb. 4, 2014, granted, now 9,068,210.
Application 14/172,326 is a continuation of application No. 12/529,926, granted, now 9,029,085, previously published as PCT/US2008/003185, filed on Mar. 7, 2008.
Claims priority of provisional application 60/905,567, filed on Mar. 7, 2007.
Prior Publication US 2025/0011827 A1, Jan. 9, 2025
This patent is subject to a terminal disclaimer.
Int. Cl. C12P 19/34 (2006.01); B01F 23/41 (2022.01); B01F 33/3011 (2022.01); B01F 33/302 (2022.01); B01J 13/00 (2006.01); C12Q 1/6806 (2018.01); C12Q 1/6834 (2018.01); C12Q 1/6848 (2018.01); C12Q 1/686 (2018.01); G01N 15/10 (2024.01); G01N 15/1404 (2024.01); B01F 101/23 (2022.01); B01L 3/00 (2006.01)
CPC C12P 19/34 (2013.01) [B01F 23/4105 (2022.01); B01F 33/3011 (2022.01); B01F 33/3021 (2022.01); B01J 13/0052 (2013.01); B01J 13/0065 (2013.01); C12Q 1/6806 (2013.01); C12Q 1/6834 (2013.01); C12Q 1/6848 (2013.01); C12Q 1/686 (2013.01); G01N 15/1404 (2013.01); B01F 23/41 (2022.01); B01F 2101/23 (2022.01); B01L 3/502784 (2013.01); C12Q 2563/149 (2013.01); C12Q 2563/159 (2013.01); G01N 2015/1006 (2013.01)] 20 Claims
 
1. A method, comprising:
(a) providing:
(i) a plurality of hardened gel droplets comprising nucleic acid molecules;
(ii) a plurality of droplets, wherein a droplet of the plurality of droplets comprises a hardened gel droplet of the plurality of hardened gel droplets, and wherein the hardened gel droplet comprises a nucleic acid molecule of the nucleic acid molecules, and
(iii) a plurality of cells, wherein a cell of the plurality of cells, comprises a sample nucleic acid molecule; and
(b) using the nucleic acid molecule and the sample nucleic acid molecule to generate a nucleic acid product.