US 12,416,005 B2
Pig with a genetically modified CD163 gene resistant to PRRSv
Andrew Mark Cigan, Madison, WI (US); Jonathan Edward Lightner, Jefferson, WI (US); Matthew Scott Culbertson, Wilmington, NC (US); William Thomas Christianson, Hendersonville, TN (US); Benjamin Beaton, DeForest, WI (US); Brian Burger, Madison, WI (US); Dylan Barnes, Madison, WI (US); and Matthew Campbell, Madison, WI (US)
Assigned to Genus plc, DeForest, WI (US)
Filed by Genus PLC, DeForest, WI (US)
Filed on Oct. 29, 2021, as Appl. No. 17/515,139.
Application 17/515,139 is a continuation of application No. 17/307,369, filed on May 4, 2021, granted, now 11,208,659.
Claims priority of provisional application 63/021,370, filed on May 7, 2020.
Claims priority of provisional application 63/020,128, filed on May 5, 2020.
Prior Publication US 2022/0042017 A1, Feb. 10, 2022
This patent is subject to a terminal disclaimer.
Int. Cl. A01K 67/027 (2024.01); A01K 67/0275 (2024.01); C12N 9/22 (2006.01); C12N 15/113 (2010.01); C12N 15/877 (2010.01)
CPC C12N 15/113 (2013.01) [A01K 67/0275 (2013.01); C12N 9/22 (2013.01); C12N 15/8778 (2013.01); A01K 2217/052 (2013.01); A01K 2227/108 (2013.01); C12N 2310/20 (2017.05); C12N 2320/34 (2013.01)] 3 Claims
 
1. A method of screening comprising:
i) breeding a genetically edited pig whose genome comprises a CD163 gene that has a homozygous deletion of exon 7 and comprises the nucleic acid sequence of SEQ ID NO: 453, wherein the pig exhibits resistance to porcine reproductive and respiratory syndrome virus (PRRSV) such that offspring are obtained;
ii) screening the genomes of the offspring for the presence of a CD163 gene that has a homozygous deletion of exon 7 and comprises the nucleic acid sequence of SEQ ID NO: 453; and
iii) screening the offspring for resistance to PRRSV.