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1. A method of reacting a nuclear target nucleic acid with a transposome complex, comprising: (a) contacting a cell nucleus comprising the nuclear target nucleic acid with an aliphatic alcohol chosen from trans-1,2-cyclohexanediol, n-hexane-1,2-diol, and 1,6-hexane-diol and reacting a transposome complex with the nuclear target nucleic acid, wherein reacting comprises fragmenting the nuclear target nucleic acid into double-stranded nucleic acid fragments, each having a 3′ end and a 5′ end, and (b) tagging the double-stranded nucleic acid fragments with the first adaptor sequence at the 5′ end of the first strand of the double-stranded nucleic acid fragments and the second adaptor sequence at the 5′ end of second strand of the double-stranded nucleic acid fragments to form tagged double-stranded nuclear nucleic acid fragments, wherein the transposome complex comprises: (i) a transposase enzyme, (ii) a first transposon end composition comprising a first transposon end sequence comprising a first adaptor sequence, and (iii) a second transposon end composition comprising a second transposon end sequence comprising a second adaptor sequence, and wherein the transposase enzyme is non-covalently bound to the first transposon end composition and the second transposon end composition.
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