US 12,416,001 B2
Nucleobase editors and methods of use thereof
Renzhi Han, Columbus, OH (US); Li Xu, Bedford, MA (US); and Yandi Gao, Powell, OH (US)
Assigned to Ohio State Innovation Foundation, Columbus, OH (US)
Appl. No. 17/799,440
Filed by Ohio State Innovation Foundation, Columbus, OH (US)
PCT Filed Feb. 12, 2021, PCT No. PCT/US2021/017868
§ 371(c)(1), (2) Date Aug. 12, 2022,
PCT Pub. No. WO2021/163492, PCT Pub. Date Aug. 19, 2021.
Claims priority of provisional application 62/976,590, filed on Feb. 14, 2020.
Prior Publication US 2023/0116627 A1, Apr. 13, 2023
Int. Cl. C12N 15/00 (2006.01); C12N 9/22 (2006.01); C12N 9/78 (2006.01); C12N 15/10 (2006.01); C12N 15/11 (2006.01); C12N 15/86 (2006.01)
CPC C12N 15/102 (2013.01) [C12N 9/22 (2013.01); C12N 9/78 (2013.01); C12N 15/11 (2013.01); C12N 15/86 (2013.01); C12N 2310/20 (2017.05); C12N 2750/14143 (2013.01); C12N 2800/40 (2013.01); C12Y 305/04002 (2013.01); C12Y 305/04005 (2013.01)] 15 Claims
OG exemplary drawing
 
1. A fusion protein comprising a Cas9 nickase and a monomeric adenine deaminase,
wherein the Cas9 nickase comprises amino acid substitutions L1111R, D1135V, G1218R, E1219F, A1322R, and T1337R, and at least one additional mutation selected from R1335V, R1335Q or R1335E when compared to SEQ ID NO: 11, and
wherein the monomeric adenine deaminase comprises amino acid substitutions A56G and V82G when compared to SEQ ID NO: 47.