US 12,415,972 B2
Alpha-amylase variants and polynucleotides encoding same
Carsten Andersen, Vaerloese (DK); Chakshusmathi Ghadiyaram, Bangalore (IN); Rajendra Kulothungan Sainathan, Bangalore (IN); Padmavathi Balumuri, Chennai (IN); Padma Venkatachalam Iyer, Mumbai (IN); Iben Damager, Valby (DK); Astrid Munch, Frederiksberg (DK); and Sohel Dalal, Ahmedabad (IN)
Assigned to Novozymes A/S, Bagsvaerd (DK)
Filed by Novozymes A/S, Bagsvaerd (DK)
Filed on Mar. 5, 2024, as Appl. No. 18/595,600.
Application 18/595,600 is a division of application No. 17/702,431, filed on Mar. 23, 2022, granted, now 11,952,557.
Application 17/702,431 is a division of application No. 16/847,773, filed on Apr. 14, 2020, granted, now 11,319,509, issued on May 3, 2022.
Application 16/847,773 is a division of application No. 15/571,219, granted, now 10,647,946, issued on May 12, 2020, previously published as PCT/EP2016/060266, filed on May 9, 2016.
Claims priority of application No. 2335/CHE/2015 (IN), filed on May 8, 2015.
Prior Publication US 2024/0271064 A1, Aug. 15, 2024
This patent is subject to a terminal disclaimer.
Int. Cl. C11D 3/386 (2006.01); C12N 9/26 (2006.01); C12N 9/28 (2006.01)
CPC C11D 3/386 (2013.01) [C12N 9/2414 (2013.01); C12N 9/2417 (2013.01); C12Y 302/01001 (2013.01)] 27 Claims
 
1. A variant of a parent alpha-amylase having alpha-amylase activity, which:
a) is a polypeptide having at least 85% sequence identity to SEQ ID NO: 13 or SEQ ID NO: 14, and
b) comprises a substitution or a deletion at a position corresponding to N195, N54, V56, G109, R116, or Q172 of the amino acid sequence as set forth in SEQ ID NO: 13 or SEQ ID NO: 14, and comprises a substitution and/or deletion in two, three or four positions corresponding to positions R181, G182, D183, and G184 of the amino acid sequence as set forth in SEQ ID NO: 13 or SEQ ID NO: 14,
c) has alpha-amylase activity, and
d) has an improved wash performance at a low temperature of 5° C. to 40° C., wherein the improved wash performance is an Improvement Factor (IF) of >1, when compared to the parent alpha-amylase.