| CPC C07K 1/20 (2013.01) | 4 Claims |
|
1. A method for purification of a synthetic peptide comprising the steps of:
performing a solid phase peptide synthesis (SPPS), wherein individual amino acids are coupled to each other via Fmoc-chemistry, wherein after at least one coupling step a capping and capture reagent is used for capping a failure peptide sequence, said capping and capture (cap-capt) reagent comprising a capping moiety and a capture moiety, and wherein said capping moiety is a carboxylic acid or an activated carboxylic acid and wherein said capture moiety is a protected carbonyl group, the capping and capture reagent being selected from
 and
 cleaving the compounds thus synthesized from the solid phase by adding trifluoroacetic acid (TFA) or TFA combined with one or more scavengers, wherein said one or more scavengers are selected from water, a silane, a thiol, a sulfide, and/or an ether,
deprotecting the capture moiety using trifluoroacetic acid (TFA) or TFA combined with one or more scavengers, wherein said one or more scavengers are selected from water, a silane, a thiol, a sulfide, and/or an ether, and
binding the deprotected capture moiety to a solid resin support thereby binding the at least one failure peptide sequence to the solid resin support and
separating the synthetic peptide from the solid resin support comprising the failure peptide.
|