	US 12,087,399 B2
	Systems and methods for customizing cell culture media for optimized cell proliferation based on genetic traits of cells
Robin Y. Smith, Boston, MA (US); and Marcie A. Glicksman, Boston, MA (US)
Assigned to Sapphiros AI Bio LLC, Boston, MA (US)
Appl. No. 16/482,946
Filed by Sapphiros AI Bio LLC, Boston, MA (US)
PCT Filed Feb. 1, 2018, PCT No. PCT/US2018/016382 § 371(c)(1), (2) Date Aug. 1, 2019, PCT Pub. No. WO2018/144693, PCT Pub. Date Aug. 9, 2018.
Claims priority of provisional application 62/453,274, filed on Feb. 1, 2017.
Prior Publication US 2020/0013481 A1, Jan. 9, 2020
Int. Cl. G16B 20/20 (2019.01); C12N 5/00 (2006.01); C12Q 1/6881 (2018.01)

CPC G16B 20/20 (2019.02) [C12N 5/0018 (2013.01); C12Q 1/6881 (2013.01); C12N 2500/30 (2013.01); C12N 2500/32 (2013.01); C12N 2500/36 (2013.01); C12N 2500/38 (2013.01); C12Q 2600/156 (2013.01); Y10S 423/05 (2013.01)]

18 Claims

1. A method comprising:

accessing, by a processor of a computing device, genotyping data corresponding to a population of cells, said genotyping data informative of one or more genetic traits of the population of cells, wherein the one or more genetic traits comprise at least one member selected from the group consisting of apoptosis, proliferation, genome instability, and acidosis,

wherein the genotyping data comprises measurements of one or more single-nucleotide polymorphisms (SNPs), wherein the one or more SNPs affect a cellular function encoded by a cellular maintenance gene or a cell cycle gene;

automatically identifying, by the processor, a composition for a cell culture medium based at least in part on the genotyping data, said composition selected for advantageous growth of the population of cells; and

contacting the population of cells with the cell culture medium to grow the population of cells.