US 12,085,557 B2
Fabrication of 3D microelectrodes and use thereof in multi-functional biosystems
Swaminathan Rajaraman, Winter Park, FL (US); Lei Zhai, Oviedo, FL (US); Avra Kundu, Orlando, FL (US); and Nilab Azim, Ovideo, FL (US)
Assigned to UNIVERSITY OF CENTRAL FLORIDA RESEARCH FOUNDATION, INC., Orlando, FL (US)
Filed by UNIVERSITY OF CENTRAL FLORIDA RESEARCH FOUNDATION, INC., Orlando, FL (US)
Filed on May 6, 2019, as Appl. No. 16/404,729.
Claims priority of provisional application 62/667,589, filed on May 6, 2018.
Prior Publication US 2019/0360995 A1, Nov. 28, 2019
Int. Cl. G01N 33/48 (2006.01); B01L 3/00 (2006.01); B29C 64/124 (2017.01); B33Y 10/00 (2015.01); C12M 1/34 (2006.01); C12M 3/00 (2006.01); C12N 1/38 (2006.01); C12N 13/00 (2006.01); D01D 5/00 (2006.01); G01N 27/12 (2006.01); G01N 27/27 (2006.01); G01N 33/483 (2006.01); C08K 3/08 (2006.01); H01L 23/29 (2006.01)
CPC G01N 33/4836 (2013.01) [B01L 3/5085 (2013.01); B29C 64/124 (2017.08); B33Y 10/00 (2014.12); C12M 1/3407 (2013.01); C12M 3/00 (2013.01); C12M 41/46 (2013.01); C12N 1/38 (2013.01); C12N 13/00 (2013.01); D01D 5/0007 (2013.01); G01N 27/128 (2013.01); G01N 27/27 (2013.01); B01L 2300/0645 (2013.01); C08K 2003/0806 (2013.01); H01L 23/293 (2013.01); Y10T 428/24339 (2015.01)] 13 Claims
OG exemplary drawing
 
1. A microelectrode platform comprising:
a platform comprising a plurality of microtower electrodes, wherein each of the plurality of microtower electrodes comprises a tip,
a metallic layer disposed on each of the plurality of microtower electrodes;
a biocompatible layer disposed on the platform, wherein the biocompatible layer comprises a plurality of apertures arranged to correspond with the tip of each of the plurality of microtower electrodes; and
a plurality of interconnected nano-scaffolds disposed in a three-dimensional pattern above the tip of each of the plurality of microtower electrodes, wherein the interconnected nano-scaffolds comprise radially aligned fibers extending out from the tip of at least one microtower electrode, and
wherein a first impedance of the microtower electrodes with nano-scaffolds is within a ten percent variance relative to a second impedance of the microtower electrodes without nanoscaffolds, when the first impedance and the second impedance are measured at the same frequency selected from a range of 800 Hz to 1000 KHz.