US 12,085,545 B2
Adjusting separation method using sensor data and numerical analysis
Bettina Schuhn, Karlsruhe (DE); and Uwe Effelsberg, Karlsruhe (DE)
Assigned to Agilent Technologies, Inc., Santa Clara, CA (US)
Filed by Agilent Technologies, Inc., Santa Clara, CA (US)
Filed on Mar. 29, 2021, as Appl. No. 17/215,872.
Claims priority of application No. 2004572.0 (GB), filed on Mar. 30, 2020.
Prior Publication US 2021/0302399 A1, Sep. 30, 2021
Int. Cl. G01N 30/86 (2006.01); G01N 30/16 (2006.01); G01N 30/02 (2006.01)
CPC G01N 30/8658 (2013.01) [G01N 30/16 (2013.01); G01N 2030/027 (2013.01)] 18 Claims
OG exemplary drawing
 
1. A process of determining a modified separation method for a sample separation apparatus based on an initial separation method, the process comprising:
carrying out the initial separation method on a sample separation apparatus by flowing a mobile phase through the sample separation apparatus in a gradient mode wherein a composition of the mobile phase is varied over time, and injecting a fluidic sample into the mobile phase, wherein the sample separation apparatus separates different components of the fluidic sample while the fluidic sample flows with the mobile phase;
detecting sensor data at the sample separation apparatus during carrying out the initial separation method, wherein the sensor data is indicative of the composition of the mobile phase flowing through the sample separation apparatus during carrying out the initial separation method on the sample separation apparatus; and
carrying out a numerical analysis for determining the modified separation method by modifying at least one operation parameter of the initial separation method and by using the detected sensor data.