US 12,085,532 B2
Enhanced chloride selective membrane
Bo Peng, Boulder, CO (US); and David Thompson, Kennett Square, PA (US)
Assigned to Siemens Healthcare Diagnostics Inc., Tarrytown, NY (US)
Filed by Siemens Healthcare Diagnostics Inc., Tarrytown, NY (US)
Filed on Jul. 24, 2023, as Appl. No. 18/357,992.
Application 18/357,992 is a continuation of application No. 17/652,937, filed on Mar. 1, 2022, granted, now 11,747,297.
Application 17/652,937 is a continuation of application No. 15/572,000, granted, now 11,293,892, issued on Apr. 5, 2022, previously published as PCT/US2016/032814, filed on May 17, 2016.
Claims priority of provisional application 62/162,843, filed on May 18, 2015.
Prior Publication US 2023/0366850 A1, Nov. 16, 2023
Int. Cl. G01N 27/333 (2006.01); G01N 27/30 (2006.01); G01N 27/414 (2006.01)
CPC G01N 27/3335 (2013.01) [G01N 27/301 (2013.01); G01N 27/414 (2013.01)] 9 Claims
 
1. A process of detecting chloride in at least one biological sample, the process comprising the steps of:
contacting a chloride selective electrode with at least one biological sample, wherein the chloride selective electrode comprises a chloride selective membrane provided with a plurality of quaternary functional groups, and wherein the chloride selective membrane comprises:
an epoxide-based matrix comprising an epoxy resin having a predetermined number of epoxide groups per molecule; and
a mixture of an amino compound and an activator dispensed onto a surface of the epoxide-based matrix, wherein the amino compound and activator are present in the mixture in a stoichiometric amount relative to the number of epoxide groups in the epoxide-based matrix, and wherein the stoichiometric amount is in a range of from about 0.5:1 to about 1.5:1, and wherein the mixture comprises reactive species; and
determining that chloride is present in the at least one biological sample based on detection of a potentiometric signal generated upon reaction between the quaternary ammonium sites on the chloride selective membrane and chloride present in the at least one biological sample.