US 12,085,495 B2
Calculating molar mass values of components of and molar mass concentration values of conjugate molecules/particles
Michelle H. Chen, Goleta, CA (US); and Daniel I. Some, Atlit (IL)
Assigned to Wyatt Technology, LLC, Goleta, CA (US)
Filed by Wyatt Technology, LLC, Goleta, CA (US)
Filed on Jun. 18, 2021, as Appl. No. 17/352,253.
Claims priority of provisional application 63/041,110, filed on Jun. 18, 2020.
Prior Publication US 2021/0396642 A1, Dec. 23, 2021
Int. Cl. G01N 15/06 (2024.01); G01N 15/075 (2024.01); G01N 15/1434 (2024.01)
CPC G01N 15/06 (2013.01) [G01N 15/1434 (2013.01); G01N 15/075 (2024.01)] 20 Claims
OG exemplary drawing
 
1. A method for using an ultraviolet (UV) light signal to analyze a biological solution, comprising:
separating, by a separation device, a plurality of conjugate molecules of the biological solution having different sizes from each other;
analyzing, by a light scattering instrument, each of the separated conjugate molecules, wherein for a molecule of the plurality of conjugate molecules, the method further comprises:
receiving, by a computer system, ΔRI values from a differential refractive index (dRI) detector, ΔUV values from a wavelength absorption (UV) detector, wherein a ΔUV value of the ΔUV values includes a change in a UV extinction at a wavelength of the biological solution relative to a pure solvent due to the molecule, and reduced Rayleigh ratio, R(θ), values from the light scattering instrument, for non-wavelength absorbing molecules/particles of the molecules, wherein the UV extinction includes an absorbance component and a scattering component;
wherein the non-wavelength absorbing molecules/particles range in size from a lower size limit to an upper size limit and are separated according to size by the separation device;
calculating, by the computer system, apparent scattering extinction coefficient values of the non-wavelength absorbing molecules/particles, sEC, and molar mass values of the non-wavelength absorbing molecules/particles, M, with respect to the ΔRI values, the ΔUV values, and the R(θ) values;
executing, by the computer system, a set of logical operations fitting the sEC values and the M values to a fit equation,
resulting in a correlation between the sEC values and the M values,
resulting in a fit function, sEC=f(M), for the non-wavelength absorbing molecules/particles, wherein: the correlation and the fit function provide for the removal of the scattering component of the UV extinction; and
applying, by the light scattering instrument, a corrected UV signal due to the removal of the scattering component of the UV extinction to analyze the each of the separated conjugate molecules.