CPC G01N 15/06 (2013.01) [G01N 15/1434 (2013.01); G01N 15/075 (2024.01)] | 20 Claims |
1. A method for using an ultraviolet (UV) light signal to analyze a biological solution, comprising:
separating, by a separation device, a plurality of conjugate molecules of the biological solution having different sizes from each other;
analyzing, by a light scattering instrument, each of the separated conjugate molecules, wherein for a molecule of the plurality of conjugate molecules, the method further comprises:
receiving, by a computer system, ΔRI values from a differential refractive index (dRI) detector, ΔUV values from a wavelength absorption (UV) detector, wherein a ΔUV value of the ΔUV values includes a change in a UV extinction at a wavelength of the biological solution relative to a pure solvent due to the molecule, and reduced Rayleigh ratio, R(θ), values from the light scattering instrument, for non-wavelength absorbing molecules/particles of the molecules, wherein the UV extinction includes an absorbance component and a scattering component;
wherein the non-wavelength absorbing molecules/particles range in size from a lower size limit to an upper size limit and are separated according to size by the separation device;
calculating, by the computer system, apparent scattering extinction coefficient values of the non-wavelength absorbing molecules/particles, sEC, and molar mass values of the non-wavelength absorbing molecules/particles, M, with respect to the ΔRI values, the ΔUV values, and the R(θ) values;
executing, by the computer system, a set of logical operations fitting the sEC values and the M values to a fit equation,
resulting in a correlation between the sEC values and the M values,
resulting in a fit function, sEC=f(M), for the non-wavelength absorbing molecules/particles, wherein: the correlation and the fit function provide for the removal of the scattering component of the UV extinction; and
applying, by the light scattering instrument, a corrected UV signal due to the removal of the scattering component of the UV extinction to analyze the each of the separated conjugate molecules.
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