US 12,084,728 B2
Increasing severity acid hydrolysis assays for determining the amount of glucose derivable from cellulose in feedstocks
Kristin Y. Nose Crotty, Palo Alto, CA (US); Bradley R. Kelemen, Palo Alto, CA (US); Colin Fritz, Decatur, IL (US); Erin Rockafellow, Decatur, IL (US); and Jacob Summerlott, Decator, IL (US)
Assigned to Danisco US Inc., Palo Alto, CA (US); and Archer Daniels Midland Company, Decatur, IL (US)
Appl. No. 17/286,699
Filed by DANISCO US INC, Palo Alto, CA (US); and ARCHER DANIELS MIDLAND COMPANY, Decatur, IL (US)
PCT Filed Oct. 14, 2019, PCT No. PCT/US2019/056071
§ 371(c)(1), (2) Date Apr. 19, 2021,
PCT Pub. No. WO2020/081432, PCT Pub. Date Apr. 23, 2020.
Claims priority of provisional application 62/747,443, filed on Oct. 18, 2018.
Prior Publication US 2021/0340638 A1, Nov. 4, 2021
Int. Cl. C12P 19/02 (2006.01); C12P 19/14 (2006.01); C13K 1/02 (2006.01); C13K 1/06 (2006.01)
CPC C13K 1/02 (2013.01) [C12P 19/02 (2013.01); C12P 19/14 (2013.01); C13K 1/06 (2013.01)] 18 Claims
OG exemplary drawing
 
1. A method for measuring an amount of cellulose-derivable glucose in a feedstock comprising a mixture of starch and cellulosic components, comprising:
dividing the feedstock into a first portion and a second portion;
contacting the first portion of the feedstock with about 30 to about 55 wt % sulfuric acid for about 1 hour, optionally at about 30° C., followed by
diluting the first portion of the feedstock to 2 wt % sulfuric acid and continuing incubation for about 45 minutes under autoclave conditions,
determining an amount of non-cellulose derivable glucose present in the first portion;
contacting the second portion of the feedstock with about 63-83 wt % sulfuric acid for about 1 hour at about 30° C., followed by
diluting the second portion of the feedstock to 4 wt % sulfuric acid and continuing incubation for about 45 minutes under autoclave conditions;
determining an amount of total glucose in the second portion; and
comparing the amount of non-cellulosic-derivable glucose determined in first portion with the amount of total glucose determined in the second portion to determine the amount of cellulose-derivable glucose in the feedstock, where an increased amount of glucose in the total glucose in the second portion of the feedstock contacted with 63-83 wt % sulfuric acid compared to the amount of glucose in the non-cellulose derivable glucose in the first portion of the feedstock contacted with about 30 to about 55 wt % sulfuric acid corresponds to cellulose-derivable glucose in the feedstock.