US 12,084,716 B2
Methods and systems for processing polynucleotides
Phillip Belgrader, Livermore, CA (US); Zachary Bent, Pleasanton, CA (US); Rajiv Bharadwaj, Pleasanton, CA (US); Vijay Kumar Sreenivasa Gopalan, Pleasanton, CA (US); Josephine Harada, San Francisco, CA (US); Christopher Hindson, Livermore, CA (US); Mohammad Rahimi Lenji, Pleasanton, CA (US); Michael Ybarra Lucero, South San Francisco, CA (US); Geoffrey McDermott, Livermore, CA (US); Elliott Meer, Pleasanton, CA (US); Tarjei Sigurd Mikkelsen, Dublin, CA (US); Christopher Joachim O'Keeffe, Pleasanton, CA (US); Katherine Pfeiffer, Berkeley, CA (US); Andrew D. Price, Hayward, CA (US); Paul Ryvkin, San Jose, CA (US); Serge Saxonov, Oakland, CA (US); John R. Stuelpnagel, Santa Barbara, CA (US); Jessica Michele Terry, Pleasanton, CA (US); Tobias Daniel Wheeler, Alameda, CA (US); Indira Wu, San Carlos, CA (US); Solongo Batjargal Ziraldo, San Ramon, CA (US); Stephane Claude Boutet, Burlingame, CA (US); Sarah Taylor, Pleasanton, CA (US); and Niranjan Srinivas, Dublin, CA (US)
Assigned to 10X GENOMICS, INC., Pleasanton, CA (US)
Filed by 10X Genomics, Inc., Pleasanton, CA (US)
Filed on Sep. 13, 2021, as Appl. No. 17/473,760.
Application 17/473,760 is a continuation of application No. 17/084,481, filed on Oct. 29, 2020, granted, now 11,180,805.
Application 17/084,481 is a continuation of application No. 16/443,638, filed on Jun. 17, 2019, granted, now 10,858,702, issued on Dec. 8, 2020.
Application 16/443,638 is a continuation of application No. 16/439,675, filed on Jun. 12, 2019, granted, now 10,815,525, issued on Oct. 27, 2020.
Application 16/439,675 is a continuation in part of application No. 15/933,299, filed on Mar. 22, 2018, granted, now 10,480,029, issued on Nov. 19, 2019.
Application 15/933,299 is a continuation of application No. 15/720,085, filed on Sep. 29, 2017, granted, now 10,011,872, issued on Jul. 3, 2018.
Application 16/439,675 is a continuation in part of application No. PCT/US2017/068320, filed on Dec. 22, 2017.
Application PCT/US2017/068320 is a continuation in part of application No. 15/720,085, filed on Sep. 29, 2017, granted, now 10,011,872, issued on Jul. 3, 2018.
Application 16/439,675 is a continuation in part of application No. PCT/US2018/064600, filed on Dec. 7, 2018.
Application PCT/US2018/064600 is a continuation in part of application No. 16/107,685, filed on Aug. 21, 2018, abandoned.
Application 16/439,675 is a continuation in part of application No. 16/107,685, filed on Aug. 21, 2018, abandoned.
Claims priority of provisional application 62/723,960, filed on Aug. 28, 2018.
Claims priority of provisional application 62/596,557, filed on Dec. 8, 2017.
Claims priority of provisional application 62/438,341, filed on Dec. 22, 2016.
Prior Publication US 2022/0106641 A1, Apr. 7, 2022
This patent is subject to a terminal disclaimer.
Int. Cl. C12Q 1/68 (2018.01); C12Q 1/6806 (2018.01); C12Q 1/683 (2018.01); C12Q 1/6874 (2018.01)
CPC C12Q 1/6874 (2013.01) [C12Q 1/6806 (2013.01); C12Q 1/683 (2013.01); C12Q 2525/191 (2013.01); C12Q 2537/143 (2013.01); C12Q 2563/179 (2013.01)] 26 Claims
 
1. A method, comprising:
(a) providing:
(i) a first molecule comprising (1) a lipophilic moiety and (2) a first nucleic acid barcode molecule, wherein the first nucleic acid barcode molecule comprises a first nucleic acid barcode sequence, and
(ii) a second molecule comprising a second nucleic acid barcode molecule, wherein the second nucleic acid barcode molecule comprises a second nucleic acid barcode sequence; and
(b) using the first nucleic acid barcode molecule and the second nucleic acid barcode molecule to generate a barcoded nucleic acid molecule comprising (1) the first nucleic acid barcode sequence or a reverse complement thereof and (2) the second nucleic acid barcode sequence or a reverse complement thereof.