	US 12,084,713 B2
	Multiplexed profiling of RNA and DNA modifications
Gudrun Stengel, San Diego, CA (US); Byron Purse, San Diego, CA (US); Yu-Hsien Hwang-Fu, San Diego, CA (US); and Jerome Santos, San Diego, CA (US)
Assigned to Alida Biosciences Inc., San Diego, CA (US)
Filed by Alida Biosciences Inc., San Diego, CA (US)
Filed on Mar. 28, 2022, as Appl. No. 17/706,470.
Application 17/706,470 is a continuation of application No. PCT/US2021/060829, filed on Nov. 24, 2021.
Claims priority of provisional application 63/193,402, filed on May 26, 2021.
Claims priority of provisional application 63/118,409, filed on Nov. 25, 2020.
Prior Publication US 2022/0298542 A1, Sep. 22, 2022
Int. Cl. C12Q 1/6804 (2018.01)

CPC C12Q 1/6804 (2013.01)

30 Claims

1. A nucleic acid-binding molecule comprising:

a binding domain that binds specifically to a non-canonical feature of a DNA or an RNA and the binding domain is selected from a reader protein, a writer protein, an eraser protein, an engineered protein scaffold, a selective covalent capture reagent, or a fragment or derivative thereof, and

an adapter,

wherein the adapter comprises a nucleic acid barcode sequence unique to the non-canonical feature bound specifically by the binding domain,

wherein the binding domain is not an antibody, and

wherein the binding domain that binds specifically to a non-canonical feature of a DNA or an RNA is coupled to a substrate.