US 12,084,712 B2
Sample indexing for single cells
Christina Chang, San Jose, CA (US); Christina Fan, San Jose, CA (US); Eleen Shum, San Jose, CA (US); Jody Martin, San Jose, CA (US); Nidhanjali Bansal, San Jose, CA (US); James Ghadiali, San Jose, CA (US); Katherine Lazaruk, San Jose, CA (US); and Gretchen Yinbon Lam, San Jose, CA (US)
Assigned to Becton, Dickinson and Company, Franklin Lakes, NJ (US)
Filed by BECTON, DICKINSON AND COMPANY, Franklin Lakes, NJ (US)
Filed on Apr. 14, 2020, as Appl. No. 16/848,241.
Application 16/848,241 is a continuation of application No. 15/937,713, filed on Mar. 27, 2018, granted, now 10,676,779.
Claims priority of provisional application 62/645,703, filed on Mar. 20, 2018.
Claims priority of provisional application 62/578,957, filed on Oct. 30, 2017.
Claims priority of provisional application 62/554,425, filed on Sep. 5, 2017.
Claims priority of provisional application 62/532,949, filed on Jul. 14, 2017.
Claims priority of provisional application 62/532,905, filed on Jul. 14, 2017.
Claims priority of provisional application 62/532,971, filed on Jul. 14, 2017.
Claims priority of provisional application 62/515,285, filed on Jun. 5, 2017.
Prior Publication US 2020/0385780 A1, Dec. 10, 2020
Int. Cl. C12Q 1/6804 (2018.01); C12Q 1/6806 (2018.01); C12Q 1/6809 (2018.01); C12Q 1/6811 (2018.01); C12Q 1/6816 (2018.01); C12Q 1/6855 (2018.01); C12Q 1/6865 (2018.01); C12Q 1/6869 (2018.01); C12Q 1/6876 (2018.01); G01N 33/532 (2006.01); B82Y 5/00 (2011.01); C12Q 1/6834 (2018.01); C12Q 1/6837 (2018.01)
CPC C12Q 1/6804 (2013.01) [C12Q 1/6806 (2013.01); C12Q 1/6809 (2013.01); C12Q 1/6811 (2013.01); C12Q 1/6816 (2013.01); C12Q 1/6855 (2013.01); C12Q 1/6865 (2013.01); C12Q 1/6869 (2013.01); C12Q 1/6876 (2013.01); G01N 33/532 (2013.01); B82Y 5/00 (2013.01); C12Q 1/6834 (2013.01); C12Q 1/6837 (2013.01); C12Q 2563/149 (2013.01); C12Q 2563/179 (2013.01); C12Q 2563/185 (2013.01); C12Q 2600/16 (2013.01); G01N 2458/10 (2013.01)] 26 Claims
OG exemplary drawing
 
1. A method for cell identification, comprising:
(a) contacting a first plurality of cells and a second plurality of cells with two sample indexing compositions, respectively,
wherein each of the first plurality of cells and each of the second plurality of cells comprises one or more cellular component targets,
wherein each of the two sample indexing compositions comprises a cellular component binding reagent associated with a sample indexing oligonucleotide,
wherein the cellular component binding reagent is capable of specifically binding to at least one of the one or more cellular component targets, and
wherein the sample indexing oligonucleotide comprises a sample indexing sequence, and wherein sample indexing sequences of the two sample indexing compositions comprise different sequences;
(b) barcoding the sample indexing oligonucleotides using a plurality of barcodes to create a plurality of barcoded sample indexing oligonucleotides,
wherein the plurality of barcodes each comprises a cell label sequence, and wherein at least two of the plurality of barcodes comprise an identical cell label sequence;
(c) obtaining sequencing data of the plurality of barcoded sample indexing oligonucleotides;
(d) identifying a cell label sequence associated with two or more sample indexing sequences in the sequencing data obtained; and
(e) removing sequencing data associated with the cell label sequence identified in step (d) from the sequencing data obtained.